Postnikov P V, Pronina I V, Polyansky D S, Efimova Yu A, Ordzhonikidze Z G, Badtieva V A, Nikityuk D B
National Anti-Doping Laboratory (Institute), Lomonosov Moscow State University, Moscow, Russia.
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.
Bull Exp Biol Med. 2025 May 29. doi: 10.1007/s10517-025-06406-x.
Xenon and cobalt are representatives of hypoxia-inducible factor (HIF) activators stimulating erythropoiesis and are prohibited by the World Anti-Doping Agency (WADA). The expression profiles of 84 microRNAs circulating in blood plasma and involved in the regulation of the HIF-1α transcription factor and its downstream signaling pathways were analyzed in volunteers before and after intake of cobalt (II) and xenon preparations using quantitative real-time PCR and certified miRCURY LNA miRNA Focus Panel (Qiagen) for analysis of microRNA expression profiles. Intake of cobalt aspartate increased the expression of hsa-miR-15b-5p, while inhalation of the Xe/O mixture significantly increased the plasma levels of hsa-miR-378a-3p and hsa-miR-491-5p. Thus, we for the first time revealed differential expression of microRNAs circulating in plasma in response to various HIF activators. Potential microRNA markers for identification of cobalt and xenon abuse for anti-doping control are proposed.
氙气和钴是刺激红细胞生成的缺氧诱导因子（HIF）激活剂的代表，被世界反兴奋剂机构（WADA）禁止使用。在志愿者摄入钴（II）和氙气制剂之前和之后，使用定量实时PCR和经过认证的miRCURY LNA miRNA Focus Panel（Qiagen）分析血浆中循环的84种参与HIF-1α转录因子及其下游信号通路调控的微小RNA的表达谱，以分析微小RNA的表达谱。摄入天冬氨酸钴会增加hsa-miR-15b-5p的表达，而吸入Xe/O混合物会显著提高hsa-miR-378a-3p和hsa-miR-491-5p的血浆水平。因此，我们首次揭示了血浆中循环的微小RNA对各种HIF激活剂的差异表达。提出了用于反兴奋剂控制中识别钴和氙气滥用的潜在微小RNA标志物。
