Boyaghchi Sepideh, Taghvimi Arezou, Hamidi Samin, Dastmalchi Siavoush, Pakravan Asrin, Javadzadeh Yousef
Biotechnology Research Center, and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
BMC Chem. 2025 May 29;19(1):153. doi: 10.1186/s13065-025-01437-x.
Regarding the increasing trend of methamphetamine consumption in society, identifying and measuring low levels of methamphetamine in biological matrices is of great importance for clinical and forensic laboratories. Coated ionic liquid silica nanoparticles (CILSNPs) are appropriate adsorbent candidates to extract methamphetamine with high selectivity and sensitivity from complex urine matrix using the mixed hemimicelle dispersive solid-phase extraction (MHMDSPE) method. After successful characterization of synthesized CILSNPs by various techniques such as FTIR, XRD, SEM, and Zeta potential, the one-factor-at-a-time (OFAT) method was performed to optimize effective extraction parameters. HPLC-UV method was used to detect and quantify methamphetamines. The optimized extraction parameters were as follows: 400 µL of methanol, 10 min of extraction, 5 min of desorption time, stirring rate of 2,000 rpm, urine pH of 10, 40 mg of adsorbent, and 10 mL of urine volume. The calibration curve was plotted in the 50-2000 ng/mL concentration range, and the method was validated. The method's limit of detection (LOD) was calculated as 9 ng/mL. Analysis of addicted subjects for the proposed method proves that the method could be used in clinical and analytical laboratories to detect methamphetamine efficiently.
鉴于社会中甲基苯丙胺消费呈上升趋势，在生物基质中识别和测定低水平的甲基苯丙胺对临床和法医实验室至关重要。涂覆离子液体二氧化硅纳米颗粒（CILSNPs）是合适的吸附剂候选物，可使用混合半胶束分散固相萃取（MHMDSPE）方法从复杂的尿液基质中高选择性和高灵敏度地提取甲基苯丙胺。通过傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、扫描电子显微镜（SEM）和zeta电位等各种技术成功表征合成的CILSNPs后，采用单因素法（OFAT）优化有效的萃取参数。使用高效液相色谱 - 紫外检测法（HPLC - UV）检测和定量甲基苯丙胺。优化后的萃取参数如下：400 μL甲醇、10分钟萃取时间、5分钟解吸时间、2000转/分钟的搅拌速率、尿液pH值为10、40毫克吸附剂和10 mL尿液体积。在50 - 2000 ng/mL浓度范围内绘制校准曲线，并对该方法进行验证。计算出该方法的检测限（LOD）为9 ng/mL。对成瘾者进行的该方法分析证明，该方法可用于临床和分析实验室以有效检测甲基苯丙胺。
