Biotechnology Research Centre and Student Research Committee, Tabriz University of Medical Science, Tabriz, Iran.
Biotechnology Research Center and Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Iran.
J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Mar 1;1109:149-154. doi: 10.1016/j.jchromb.2019.02.005. Epub 2019 Feb 7.
Carboxylated carbon porous adsorbent was derived from zeolite imidazole framework (ZIF-8) via carbonization of ZIF-8 under a nitrogen atmosphere. The synthesized carboxylated adsorbent was fully characterized by various techniques including Fourier transform spectroscopy (FTIR), powder X-ray diffraction (XRD), scanning electron microscopy (SEM), and zeta potential analysis. The carboxylated adsorbent was applied as dispersive solid phase extraction (DSPE) adsorbent for efficient extraction of methamphetamine (MET) from biological urine samples. Several extraction parameters influencing the extraction efficiency were investigated and the calibration curve was plotted under optimized conditions in urine media. The method showed a good linearity in the range of 50-2500 ng/mL. The limit of detection (LOD) and limit of quantification (LOQ) was 10 and 35.80 ng/mL, respectively. A satisfactory analysis of the positive real samples with the recovery of 99.83% confirms the applicability of the proposed method in different clinical and forensic laboratories.
羧基化碳多孔吸附剂是通过氮气气氛下碳化沸石咪唑骨架（ZIF-8）得到的。合成的羧基化吸附剂通过多种技术进行了充分的表征，包括傅里叶变换光谱（FTIR）、粉末 X 射线衍射（XRD）、扫描电子显微镜（SEM）和 ζ 电位分析。羧基化吸附剂被用作分散固相萃取（DSPE）吸附剂，用于从生物尿液样品中高效提取甲基苯丙胺（MET）。研究了影响萃取效率的几个萃取参数，并在尿液介质中优化条件下绘制了校准曲线。该方法在 50-2500ng/mL 的范围内表现出良好的线性。检测限（LOD）和定量限（LOQ）分别为 10 和 35.80ng/mL。对阳性实际样品的满意分析，回收率为 99.83%，证实了该方法在不同临床和法医实验室中的适用性。
