盘基网柄菌变形虫细胞膜中碱性磷酸酶的细胞化学定位
Cytochemical localization of alkaline phosphatase in the cell membrane of Dictyostelium discoideum amebae.
作者信息
Glomp I, Schäfer D, Hess B
出版信息
Histochemistry. 1985;83(3):251-5. doi: 10.1007/BF00953993.
PMID:4044310
Abstract
We demonstrated that alkaline phosphatase was localized on the cell membrane of Dictyostelium discoideum amebae and on isolated plasma membranes. The enzyme activity was specifically inhibited by 0.01 M KCN or cysteine. The same method could also be applied to baker's yeast and MDCK cells (dog kidney cells in vitro).
摘要
我们证明碱性磷酸酶定位于盘基网柄菌变形虫的细胞膜以及分离出的质膜上。该酶活性受到0.01 M KCN或半胱氨酸的特异性抑制。同样的方法也可应用于面包酵母和MDCK细胞（体外培养的犬肾细胞）。
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