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盘基网柄菌变形虫细胞膜中碱性磷酸酶的细胞化学定位

Cytochemical localization of alkaline phosphatase in the cell membrane of Dictyostelium discoideum amebae.

作者信息

Glomp I, Schäfer D, Hess B

出版信息

Histochemistry. 1985;83(3):251-5. doi: 10.1007/BF00953993.

DOI:10.1007/BF00953993
PMID:4044310
Abstract

We demonstrated that alkaline phosphatase was localized on the cell membrane of Dictyostelium discoideum amebae and on isolated plasma membranes. The enzyme activity was specifically inhibited by 0.01 M KCN or cysteine. The same method could also be applied to baker's yeast and MDCK cells (dog kidney cells in vitro).

摘要

我们证明碱性磷酸酶定位于盘基网柄菌变形虫的细胞膜以及分离出的质膜上。该酶活性受到0.01 M KCN或半胱氨酸的特异性抑制。同样的方法也可应用于面包酵母和MDCK细胞（体外培养的犬肾细胞）。

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Cytochemical localization of alkaline phosphatase in the cell membrane of Dictyostelium discoideum amebae.盘基网柄菌变形虫细胞膜中碱性磷酸酶的细胞化学定位
Histochemistry. 1985;83(3):251-5. doi: 10.1007/BF00953993.
2
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Biochem J. 1988 Jan 1;249(1):223-30. doi: 10.1042/bj2490223.
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本文引用的文献

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A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY.一种用于电子显微镜的简化柠檬酸铅染色法。
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Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture.细胞黏菌盘基网柄菌的黏变形体在无菌培养中的生长。
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[Effects of 8 various aldehydes and of the pH value on the localization of glucose-6-phosphatase in cultured cells].
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The isolation and subfractionation of plasma membrane from the cellular slime mould Dictyostelium discoideum.从细胞黏菌盘基网柄菌中分离和亚分级分离质膜。
Biochem J. 1974 May;140(2):313-22. doi: 10.1042/bj1400313.
10
Cytochemical demonstration of alkaline phosphatase in the contractile vacuole of Dictyostelium discoideum.盘基网柄菌收缩泡中碱性磷酸酶的细胞化学证明
J Ultrastruct Res. 1978 Mar;62(3):228-36. doi: 10.1016/s0022-5320(78)80020-3.