Nam Eoun Ho, Lee Minjee, Kim Donggyu, Jung Young Hoon, Yang Jungwoo, Shin Minhye
Department of Microbiology, College of Medicine, Inha University, Incheon 22212, Republic of Korea.
Program in Biomedical Science and Engineering, Inha University, Incheon 22212, Republic of Korea.
J Microbiol Biotechnol. 2025 May 28;35:e2502045. doi: 10.4014/jmb.2502.02045.
Probiotics have been extensively investigated as potential food supplements for human health benefits. Metabolites derived from probiotics are the primary factors that characterize each strain's functionality and play a crucial role in shaping their effects on the human host. In this study, we characterized the secreted metabolite profiles of sixteen commercial probiotic strains and identified IDCC 2201 as a major folate producer. To investigate its effects on gut microbiota, was co-cultured with individual species comprising the human gut microbial community. Specific bacteria, such as , , and , grew dependently on both folate and . These bacteria exhibited greater growth in the presence of folate than in its absence, with 2.8-, 3.6-, and 3.9-fold increases, respectively. Additionally, they showed relatively higher growth when co-cultured with compared to other bacterial species, with 1.2-, 1.3-, and 1.9-fold increases, respectively. Our results indicate that the interaction between probiotics and the human gut microbiota can influence changes in ecological balance through nutrient cross-feeding, and understanding this interaction can be applied to precision probiotic therapies.
益生菌作为具有潜在健康益处的食品补充剂已得到广泛研究。益生菌产生的代谢产物是表征每个菌株功能的主要因素，并且在塑造它们对人类宿主的影响方面起着关键作用。在本研究中，我们表征了16种商业益生菌菌株的分泌代谢产物谱，并鉴定出IDCC 2201是主要的叶酸生产者。为了研究其对肠道微生物群的影响，将其与构成人类肠道微生物群落的各个物种共同培养。特定细菌，如[具体细菌名称1]、[具体细菌名称2]和[具体细菌名称3]，在叶酸和[另一种物质名称]的共同作用下生长。这些细菌在有叶酸存在时比没有叶酸时生长得更好，分别增加了2.8倍、3.6倍和3.9倍。此外，与其他细菌物种相比，它们与[IDCC 2201]共同培养时显示出相对更高的生长，分别增加了1.2倍、1.3倍和1.9倍。我们的结果表明，益生菌与人类肠道微生物群之间的相互作用可以通过营养交叉喂养影响生态平衡的变化，并且理解这种相互作用可应用于精准益生菌疗法。
原文中部分细菌名称和物质名称缺失，翻译时用[具体细菌名称1]、[具体细菌名称2]、[具体细菌名称3]、[另一种物质名称]等表示。
