La Paglia Laura, Mauro Manuela, Arizza Vincenzo, Urso Alfonso, Simon Sugár, Drahos Laszlo, di Stefano Vita, Luparello Claudio, Vazzana Mirella, Vizzini Aiti
Institute of High Performance Computing and Networking (ICAR)-CNR, National Research Council of Italy, Palermo, Italy.
Department of Biological and Technological Science, Chemical and Pharmaceutical Science (STEBICEF), University of Studies of Palermo, Palermo, Italy.
Front Immunol. 2025 May 15;16:1539751. doi: 10.3389/fimmu.2025.1539751. eCollection 2025.
The holothurian immune system is characterized by complex defense mechanisms that act through humoral and cellular pathways. Coelomocites are the cellular component of coelomic fluid, and they are involved in host defense, stress response, wound healing, organ regeneration, and tissue homeostasis. The close phylogenetic relationship between and chordate phylum makes it a good model for studying the evolution of immune processes. To elucidate the immune landscape in , we applied an approach combining proteomic analysis of coelomic fluid separated into cellular fraction and extracellular fraction and bioinformatics and in silico analyses. A Search Tool for the Retrieval of Interacting Genes/Protein analysis indicated a highly functional homology to the human protein of immune recognition factors, non-canonical immune-related proteins, signaling molecules, and effector protein, cytoskeleton, and actin remodeling, and provided the first evidence in invertebrate immune cells of an intracellular protein fraction linked to ancestral structure resembling primary cilium involved in cell signaling.
海参的免疫系统具有复杂的防御机制，通过体液和细胞途径发挥作用。体腔细胞是体腔液的细胞成分，参与宿主防御、应激反应、伤口愈合、器官再生和组织稳态。海参与脊索动物门之间密切的系统发育关系使其成为研究免疫过程进化的良好模型。为了阐明海参的免疫格局，我们采用了一种方法，将体腔液蛋白质组分析分为细胞部分和细胞外部分，并结合生物信息学和计算机分析。检索相互作用基因/蛋白质分析的搜索工具表明，海参与人类蛋白质在免疫识别因子、非经典免疫相关蛋白质、信号分子和效应蛋白、细胞骨架和肌动蛋白重塑方面具有高度功能同源性，并首次在无脊椎动物免疫细胞中提供了与参与细胞信号传导的类似初级纤毛的祖先结构相关的细胞内蛋白质部分的证据。
