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妊娠前半期甲状腺功能减退症的宏基因组学和蛋白质组学特征及其与Th1/Th2平衡相关性的探索性研究

An exploratory study on the metagenomic and proteomic characterization of hypothyroidism in the first half of pregnancy and correlation with Th1/Th2 balance.

作者信息

Zhang Chenchen, Xu Yajuan, Zhang Miao, Li Jingjing, Sun Zongzong, Wang Yixin, Lin Pengkun

机构信息

Department of Obstetrics and Gynecology, Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

出版信息

Front Immunol. 2025 May 15;16:1500866. doi: 10.3389/fimmu.2025.1500866. eCollection 2025.

DOI:10.3389/fimmu.2025.1500866
PMID:40443669
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12120637/
Abstract

OBJECTIVE: To explore the gut microbiota and proteomic characteristics of hypothyroidism in the first half of pregnancy (referred to as hypothyroidism in the first half of pregnancy) and its association with Th (T helper cells, Th)1/Th2 balance using metagenomics combined with proteomics. METHODS: Stool and blood samples were collected from 20 hypothyroid (hypothyroidism group) and normal pregnant women (normal group) in the first half of pregnancy. Flora and proteomic characteristics were analyzed using metagenomics sequencing and 4D-DIA proteomics. Th1 and Th2 cells were quantified, and cytokine levels were measured using cellular micro-bead arra. The enzyme-linked immunosorbent test (ELISA) was utilized to assess differential proteins. RESULTS: (1) Metagenomic sequencing revealed distinct microbial profiles: The β-diversity of gut microbiota was diminished in the hypothyroidism group (p < 0.05). LEfSe analysis identified and enriched in the hypothyroidism group (p<0.05), and Kyoto Encyclopedia of Genes and Genomes(KEGG) analysis showed significant enrichment in pathways related to peptidoglycan biosynthesis and glycerol ester metabolism.(2) Proteomic analysis demonstrated downregulation of Diacylglycerol Kinase Kappa (DGKK) and P05109|S10A8(S10A8) proteins in the hypothyroidism group, with marked enrichment in the KEGG pathways for vascular smooth muscle contraction and phosphatidylinositol signaling. (3) ELISA validation confirmed that the proteins DGKK and S10A8 were downregulated in pregnant women in the hypothyroidism group. CONCLUSION: Increased and , decreased DGKK and S10A8 proteins, and a left shift in the Th1/Th2 balance in patients with hypothyroidism in the first half of pregnancy may be associated with the development of the disease.

摘要

目的：采用宏基因组学与蛋白质组学相结合的方法，探讨妊娠前半期甲状腺功能减退症（以下简称妊娠前半期甲减）的肠道微生物群和蛋白质组学特征及其与Th（辅助性T细胞，Th）1/Th2平衡的关系。 方法：收集20例妊娠前半期甲状腺功能减退孕妇（甲减组）和正常孕妇（正常组）的粪便和血液样本。采用宏基因组学测序和4D-DIA蛋白质组学分析菌群和蛋白质组学特征。对Th1和Th2细胞进行定量，并使用细胞微珠阵列测量细胞因子水平。采用酶联免疫吸附试验（ELISA）评估差异蛋白。 结果：（1）宏基因组学测序显示出不同的微生物谱：甲减组肠道微生物群的β多样性降低（p<0.05）。LEfSe分析确定了在甲减组中富集的 和 （p<0.05），京都基因与基因组百科全书（KEGG）分析显示与肽聚糖生物合成和甘油酯代谢相关的途径有显著富集。（2）蛋白质组学分析表明，甲减组二酰甘油激酶κ（DGKK）和P05109|S10A8（S10A8）蛋白下调，在血管平滑肌收缩和磷脂酰肌醇信号传导的KEGG途径中显著富集。（3）ELISA验证证实，甲减组孕妇中DGKK和S10A8蛋白下调。 结论：妊娠前半期甲减患者 和 增加、DGKK和S10A8蛋白减少以及Th1/Th2平衡左移可能与该疾病的发生有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b9/12120637/6d29b2ff721e/fimmu-16-1500866-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b9/12120637/b162da934380/fimmu-16-1500866-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b9/12120637/c6825adf918f/fimmu-16-1500866-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b9/12120637/851c81d8723b/fimmu-16-1500866-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b9/12120637/dd3b55240736/fimmu-16-1500866-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b9/12120637/5877903caf91/fimmu-16-1500866-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b9/12120637/1797b4ed8fec/fimmu-16-1500866-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b9/12120637/6d29b2ff721e/fimmu-16-1500866-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b9/12120637/b162da934380/fimmu-16-1500866-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b9/12120637/c6825adf918f/fimmu-16-1500866-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b9/12120637/851c81d8723b/fimmu-16-1500866-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b9/12120637/dd3b55240736/fimmu-16-1500866-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b9/12120637/5877903caf91/fimmu-16-1500866-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b9/12120637/1797b4ed8fec/fimmu-16-1500866-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b9/12120637/6d29b2ff721e/fimmu-16-1500866-g007.jpg

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本文引用的文献

[1]
Biochemical markers for prediction of the first half pregnancy losses: a review.

Rev Bras Ginecol Obstet. 2024

[2]
What Are the Reliable Plasma Biomarkers for Mild Cognitive Impairment? A Clinical 4D Proteomics Study and Validation.

Mediators Inflamm. 2024

[3]
NADPH oxidase 4-derived hydrogen peroxide counterbalances testosterone-induced endothelial dysfunction and migration.

Am J Physiol Endocrinol Metab. 2024-7-1

[4]
Nardilysin in vascular smooth muscle cells controls blood pressure via the regulation of calcium dynamics.

Biochem Biophys Res Commun. 2024-6-18

[5]
Highland barley β-glucan supplementation attenuated hepatic lipid accumulation in Western diet-induced non-alcoholic fatty liver disease mice by modulating gut microbiota.

Food Funct. 2024-2-5

[6]
Portable BLAST-like algorithm library and its implementations for command line, Python, and R.

PLoS One. 2023

[7]
S100A8/A9-alarmin promotes local myeloid-derived suppressor cell activation restricting severe autoimmune arthritis.

Cell Rep. 2023-8-29

[8]
Gut microbiota, intestinal permeability, and systemic inflammation: a narrative review.

Intern Emerg Med. 2024-3

[9]
Correlation between the intestinal microflora and peripheral blood Th1/Th2 balance in hypothyroidism during the first half of pregnancy.

Front Cell Infect Microbiol. 2023

[10]
Extracellular vesicles derived from CD4 T cells carry DGKK to promote sepsis-induced lung injury by regulating oxidative stress and inflammation.

Cell Mol Biol Lett. 2023-3-23

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