Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Front Endocrinol (Lausanne). 2022 Jan 19;12:792536. doi: 10.3389/fendo.2021.792536. eCollection 2021.
To investigate the lipid profiles and intestinal microflora in pregnant patients with hypothyroidism and their correlation with pregnancy outcomes.
In total, 27 pregnant women with hypothyroidism (study case) and 28 normal pregnant women (control group) were enrolled in this study. The lipid profiles and intestinal microflora in the two groups were compared using untargeted liquid chromatography-mass spectrometry (LC-MS) and 16S rRNA amplicon sequencing, respectively. The association among the differential metabolites, intestinal microflora, serological indicators and pregnancy outcomes was further analyzed.
Patients in study case had higher C-reactive protein (CRP) levels (P = 0.025) and lower birth weight (P=0.005) than the control group. A total of 42 differential lipid metabolites and 7 enrichment KEGG pathways were obtained between the two groups (VIP ≥ 1, P < 0.05). Ten lipid metabolites can be used as characteristic metabolites of study case, including phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM). The richness and diversity of intestinal microflora in study case were lower than those in the control group (P>0.05). LEfSe analysis revealed that patients in study case had higher abundance of and and lower abundance of than the control group (P < 0.05). was positively correlated with SM and negatively correlated with PC and PE; the CRP level and were positively correlated; the neonatal weight and PC level were negatively correlated (P < 0.05).
The lipid profile and intestinal microflora of pregnant women with hypothyroidism significantly differed from those of normal pregnant women and were associated with adverse pregnancy outcomes. The interaction between lipid metabolism and intestinal microflora may be a potential target for further studies investigating the pathogenesis of hypothyroidism during pregnancy.
探讨甲状腺功能减退症孕妇的血脂谱和肠道菌群及其与妊娠结局的关系。
本研究共纳入 27 例甲状腺功能减退症孕妇（病例组）和 28 例正常孕妇（对照组）。采用非靶向液相色谱-质谱（LC-MS）和 16S rRNA 扩增子测序分别比较两组的血脂谱和肠道菌群。进一步分析差异代谢物、肠道菌群、血清学指标与妊娠结局的关系。
病例组的 C 反应蛋白（CRP）水平较高（P=0.025），出生体重较低（P=0.005）。两组间共获得 42 个差异脂质代谢物和 7 个富集的 KEGG 通路（VIP≥1，P<0.05）。10 个脂质代谢物可作为病例组的特征代谢物，包括磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）和鞘磷脂（SM）。病例组肠道菌群的丰富度和多样性均低于对照组（P>0.05）。LEfSe 分析显示，病例组的 丰度较高，而 丰度较低（P<0.05）。 与 SM 呈正相关，与 PC 和 PE 呈负相关；CRP 水平与 呈正相关；新生儿体重与 PC 水平呈负相关（P<0.05）。
甲状腺功能减退症孕妇的血脂谱和肠道菌群与正常孕妇明显不同，与不良妊娠结局有关。脂代谢与肠道菌群的相互作用可能是进一步研究甲状腺功能减退症发病机制的潜在靶点。
