Male Reproductive Physiology lab, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
Male Reproductive Physiology lab, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
Peptides. 2023 Jan;159:170899. doi: 10.1016/j.peptides.2022.170899. Epub 2022 Nov 3.
Neuromedin S (NMS) is a well-known anorexigenic neuropeptide. Despite some reports of the presence of its transcript and precursor protein in testis, the expression and localization of NMS and its receptors during the postnatal development of mammalian testis remains elusive. We investigated the expression patterns and testicular localization of NMS and its receptors NMUR1 and NMUR2, during 5, 10, 20, 30, and 90 days of postnatal development, using real time PCR, immunoblot analysis and immunohistochemistry in mice. NMS and its receptors are present at all age groups at transcript level in mouse testis. At the protein level, NMS and NMUR2 are present in all age groups, whereas NMUR1 is present primarily in 30- and 90-day testis. Immunolocalization study showed that NMS and NMUR2 are expressed in spermatogonia, spermatocytes, Sertoli cells, and Leydig cells, in contrast to NMUR1 which is expressed exclusively in the Leydig cells of 30- and 90-day testis. The results also confirm the intranuclear localization of NMS in spermatogonia and spermatocytes. Although NMS-NMUR2 is expressed in Sertoli cells at all stages of the spermatogenic cycle, they showed a stage-specific expression pattern in spermatogonia and primary spermatocytes. In conclusion, NMS and its receptors NMUR1 and NMUR2 are expressed in the testis and may regulate spermatogenesis, possibly by modulating steroidogenesis and Sertoli cell function in the testis.
神经调节素 S（NMS）是一种众所周知的厌食神经肽。尽管有一些报道称其转录本和前体蛋白存在于睾丸中，但 NMS 及其受体在哺乳动物睾丸的出生后发育过程中的表达和定位仍然难以捉摸。我们使用实时 PCR、免疫印迹分析和免疫组织化学方法，在小鼠中研究了 NMS 及其受体 NMUR1 和 NMUR2 在出生后 5、10、20、30 和 90 天的表达模式和睾丸定位。在小鼠睾丸中，NMS 和其受体在所有年龄段的转录本水平上都存在。在蛋白质水平上，NMS 和 NMUR2 存在于所有年龄段，而 NMUR1 主要存在于 30 日龄和 90 日龄的睾丸中。免疫定位研究表明，NMS 和 NMUR2 表达于精原细胞、精母细胞、支持细胞和间质细胞，而 NMUR1 仅表达于 30 日龄和 90 日龄睾丸的间质细胞中。结果还证实了 NMS 在精原细胞和精母细胞中的核内定位。虽然 NMS-NMUR2 在精子发生周期的所有阶段都在支持细胞中表达，但它们在精原细胞和初级精母细胞中表现出特定阶段的表达模式。总之，NMS 及其受体 NMUR1 和 NMUR2 在睾丸中表达，可能通过调节睾丸中的类固醇生成和支持细胞功能来调节精子发生。
