Sun Yijun, Gao Xiangyu, Huang Zhonghui, Li Can, Cheng Yuanyuan, Tian Li-Wen
School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, People's Republic of China.
Analytical and Testing Center, Jiangxi Science and Technology Normal University, Nanchang 330013, People's Republic of China.
J Nat Prod. 2025 Jun 27;88(6):1481-1490. doi: 10.1021/acs.jnatprod.5c00424. Epub 2025 May 30.
Four previously undescribed phenolic compounds, -, including a racemic stilbene dimer featuring a rare benzobicyclo[4.2.1]nonene motif (/), a racemic 1,2-diarylpropanoid (/), and an aurone (), along with 14 known metabolites, were isolated from the rhizomes of . The structures of compounds - were identified by extensive HRESIMS, NMR data analysis, X-ray diffraction crystallography, and ECD calculation. The anti-inflammatory activities of compounds - were evaluated by the lipopolysaccharide (LPS)-stimulated RAW264.7 cell model. Compounds and significantly inhibited the release of NO in LPS-stimulated RAW264.7 cells, with IC values of 5.7 and 5.3 μM, respectively. Moreover, the expression of pro-inflammatory cytokines (TNFα, IL-6, IL-1β, and NOS2) was also blocked by them in a dose-dependent manner. Mechanistically, compounds and suppressed the TLR4/NF-κB signaling pathway in RAW264.7 cells.
从[植物名称]的根茎中分离出四种先前未描述的酚类化合物，包括一种具有罕见苯并双环[4.2.1]壬烯基序的外消旋芪二聚体（/）、一种外消旋1,2 - 二芳基丙烷类化合物（/）和一种橙酮（），以及14种已知代谢物。通过广泛的高分辨电喷雾电离质谱（HRESIMS）、核磁共振（NMR）数据分析、X射线衍射晶体学和电子圆二色光谱（ECD）计算确定了化合物 - 的结构。通过脂多糖（LPS）刺激的RAW264.7细胞模型评估了化合物 - 的抗炎活性。化合物 和 显著抑制LPS刺激的RAW264.7细胞中一氧化氮（NO）的释放，IC值分别为5.7和5.3 μM。此外，它们还以剂量依赖性方式阻断促炎细胞因子（TNFα、IL - 6、IL - 1β和NOS2）的表达。从机制上讲，化合物 和 抑制RAW264.7细胞中的TLR4/NF - κB信号通路。
