用于验证rRNA基因扩增子测序的海洋微生物模拟群落
Marine microbial mock communities for validating rRNA gene amplicon sequencing.
作者信息
Lampe Robert H, Rabines Ariel J, Ellman Bryce A, Zheng Hong, Allen Andrew E
机构信息
Integrative Oceanography Division, Scripps Institution of Oceanography, University of California San Diego, La Jolla, California, USA.
Microbial and Environmental Genomics, J. Craig Venter Institute, La Jolla, California, USA.
出版信息
Microbiol Resour Announc. 2025 Jul 10;14(7):e0029825. doi: 10.1128/mra.00298-25. Epub 2025 May 30.
Mock communities are defined mixtures of cells or DNA for methods development, testing, or validation. Here, we present mock communities based on previously used 16S and 18S rRNA gene sequences from diverse marine microorganisms. Their use as controls for amplicon sequencing enables the detection of biased or aberrant runs.
模拟群落是为方法开发、测试或验证而定义的细胞或DNA混合物。在此，我们基于先前使用的来自不同海洋微生物的16S和18S rRNA基因序列呈现模拟群落。将它们用作扩增子测序的对照能够检测有偏差或异常的测序运行。
Zotero 插件