Babbo Carina C, Pitere Reabetswe R, Giles Rachel, Ambele Melvin A, Pepper Michael S
Institute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
Methods Mol Biol. 2025;2938:53-62. doi: 10.1007/978-1-0716-4607-6_6.
Adipogenesis is a complex multiphase process in which preadipocytes develop into adipocytes. This tightly regulated process is facilitated by a cascade of genes and transcription factors. In this chapter, we describe a protocol that can be used to quantify the expression of adipogenic markers using RT-qPCR.
脂肪生成是一个复杂的多阶段过程，在此过程中前脂肪细胞发育为脂肪细胞。这一受到严格调控的过程由一系列基因和转录因子促成。在本章中，我们描述了一种可用于通过逆转录定量聚合酶链反应（RT-qPCR）来量化脂肪生成标志物表达的方案。
