Motaung Thabiso Eric
Faculty of Natural and Agricultural Sciences, Department of Biochemistry, Genetics, and Microbiology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria, South Africa.
Methods Mol Biol. 2025;2938:133-143. doi: 10.1007/978-1-0716-4607-6_16.
Adipocyte-derived extracellular vesicles (AdEVs), like other types of extracellular vesicles (EVs), are released from their donor parental cells in varying sizes and large quantities. During this process, diverse cargos are often internalized in the lumen or covalently attached on the surface of these EVs, allowing for their horizontal transfer from adipocytes to non-adipocyte cells. Hence, AdEVs can promote the ability of adipocytes to remodel the tumor microenvironment in cancers affecting, among others, breast and colon tissues by acting as mediators of cell-to-cell communication through the release of bioactive molecules. Cancer-associated adipocytes, for instance, can promote tumor growth, metastasis, and therapeutic resistance by secreting AdEVs carrying oncogenic microRNAs, cytokines, and chemokines, which interact with signaling pathways (e.g., JAK/STAT3 and PI3K/AKT) that lead to tumor formation and cancerous behavior. As the importance of EVs in these processes rapidly gains attention, a reliable method of isolating AdEVs also becomes highly essential. Therefore, this chapter describes the use of size-exclusion chromatography given its ability to preserve vesicle integrity and achieve high sample purity.
脂肪细胞衍生的细胞外囊泡（AdEVs）与其他类型的细胞外囊泡（EVs）一样，以不同大小和大量从其供体亲代细胞中释放出来。在此过程中，多种货物常常被内化到这些囊泡的腔内或共价附着在其表面，从而实现它们从脂肪细胞到非脂肪细胞的水平转移。因此，AdEVs可以通过作为细胞间通讯的介质释放生物活性分子，促进脂肪细胞重塑影响乳腺和结肠组织等多种癌症的肿瘤微环境的能力。例如，癌症相关脂肪细胞可以通过分泌携带致癌微小RNA、细胞因子和趋化因子的AdEVs来促进肿瘤生长、转移和治疗抗性，这些分子与导致肿瘤形成和癌性行为的信号通路（如JAK/STAT3和PI3K/AKT）相互作用。随着细胞外囊泡在这些过程中的重要性迅速受到关注，一种可靠的分离AdEVs的方法也变得至关重要。因此，本章介绍了尺寸排阻色谱法的使用，因为它能够保持囊泡完整性并实现高样品纯度。
