Streeter J G
J Bacteriol. 1985 Oct;164(1):78-84. doi: 10.1128/jb.164.1.78-84.1985.
Four strains of Rhizobium japonicum (61A76 and USDA 110, 123, and 138) were grown in eight different defined media. Regardless of the carbon or nitrogen source supplied, alpha, alpha-trehalose was the major carbohydrate (among mono- and disaccharides) accumulated by all four strains. After 7 to 9 days of growth, trehalose generally accounted for 90 to 100% of the mono- and disaccharides detected. None of the four strains would grow with trehalose as a carbon source, but the utilization of endogenous trehalose was demonstrated under carbon starvation conditions in water culture or when the carbon supply in a defined medium was exhausted. Under these conditions, a small amount of trehalose was lost from cells to the medium. In a survey of most of the serogroups of R. japonicum and several strains of other Rhizobium species, all strains tested were found to accumulate some trehalose. Trehalose concentrations varied widely; the highest concentration recorded was 41 micrograms/mg of dry weight. In all but six strains trehalose accounted for greater than 80% of the mono- and disaccharides in cells. Fast-growing strains of R. japonicum also accumulated small amounts trehalose. R. japonicum bacteroids also synthesized trehalose; the quantity in nodules varied in approximate correspondence to accumulation of trehalose by cultured bacteria. In young soybean nodules (29 days after planting), 45 to 80% of the trehalose was recovered in the cytosol. There were differences among R. japonicum strains in the retention of trehalose, and the proportion of trehalose retained by bacteroids increased with increasing plant age for all strains.
四株日本根瘤菌（61A76以及美国农业部菌株110、123和138）在八种不同的限定培养基中培养。无论提供何种碳源或氮源，α,α-海藻糖都是这四株菌株积累的主要碳水化合物（在单糖和双糖中）。生长7至9天后，海藻糖通常占检测到的单糖和双糖的90%至100%。这四株菌株均不能以海藻糖作为碳源生长，但在水培中的碳饥饿条件下或限定培养基中的碳源耗尽时，可证明其对内生海藻糖的利用。在这些条件下，少量海藻糖从细胞中流失到培养基中。在对大多数日本根瘤菌血清群和其他几种根瘤菌属菌株的调查中，发现所有测试菌株都积累了一些海藻糖。海藻糖浓度差异很大；记录到的最高浓度为41微克/毫克干重。除六株菌株外，所有菌株中海藻糖占细胞中单糖和双糖的比例均超过80%。日本根瘤菌的快速生长菌株也积累少量海藻糖。日本根瘤菌类菌体也合成海藻糖；根瘤中的数量变化与培养细菌中海藻糖的积累大致对应。在年轻的大豆根瘤（种植后29天）中，45%至80%的海藻糖存在于细胞质中。不同日本根瘤菌菌株在海藻糖保留方面存在差异，并且所有菌株类所有菌株而言，类菌体保留的海藻糖比例随植株年龄的增加而增加。
