Cheng Yuliang, Zhao Yajie, Li Peizhen, Yu Hang, Xie Yunfei, Guo Yahui, Yao Weirong, Qian He
State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, 214122, China.
Mikrochim Acta. 2025 May 30;192(6):387. doi: 10.1007/s00604-025-07257-z.
As one of the most extensively used antibiotics in aquaculture in China, enrofloxacin (ENR) tends to accumulate in human body and endanger people's health. Rapid, sensitive, and facile assays for enrofloxacin are urgently needed. In this study, based on the solid-phase substrate preparation strategy combined with microsphere etching and magnetron sputtering, Ag@Au nanoparticle (Ag@Au NP) solid array (Ag@Au NSA) with high surface-enhanced Raman spectroscopy (SERS) enhancement factor (EF≈8.3 × 10) was developed. Finite-difference time-domain (FDTD) simulations revealed that the Ag@Au NSA achieved maximum electromagnetic enhancement (EM) under the following optimized conditions: 633 nm excitation wavelength, 7.9 nm Au shell thickness, and 30.5 nm Ag core size. Additionally, SERS mapping results confirmed both the excellent enhancement performance and signal reproducibility of Ag@Au NSA, due to the expected micro-region enhancement with long-term effects accompanied by a larger range of high-density "hot spots." The proposed SERS sensing for ENR based on Ag@Au NSA had a limit of detection (LOD) of 0.076 ng/mL (R ≈ 0. 9991) and was further applied to real-sample detection of ENR in crayfish tail meat with good recoveries ranging from 91.36 to 112.60%, indicating its great potential in practice.
恩诺沙星（ENR）是中国水产养殖中使用最广泛的抗生素之一，它容易在人体内蓄积，危害人们的健康。因此，迫切需要一种快速、灵敏且简便的恩诺沙星检测方法。在本研究中，基于固相基底制备策略，结合微球蚀刻和磁控溅射技术，制备了具有高表面增强拉曼光谱（SERS）增强因子（EF≈8.3×10）的Ag@Au纳米颗粒（Ag@Au NP）固体阵列（Ag@Au NSA）。时域有限差分（FDTD）模拟表明，在633 nm激发波长、7.9 nm金壳厚度和30.5 nm银核尺寸的优化条件下，Ag@Au NSA实现了最大电磁增强（EM）。此外，SERS映射结果证实了Ag@Au NSA具有优异的增强性能和信号重现性，这归因于预期的微区增强具有长期效应，并伴有更大范围的高密度“热点”。所提出的基于Ag@Au NSA的ENR SERS传感检测限（LOD）为0.076 ng/mL（R≈0.9991），并进一步应用于小龙虾尾肉中ENR的实际样品检测，回收率良好，在91.36%至112.60%之间，表明其在实际应用中具有巨大潜力。
