Jarczewska Karolina, Kopeć Monika, Surmacki Jakub Maciej
Lodz University of Technology, Faculty of Chemistry, Institute of Applied Radiation Chemistry, Laboratory of Laser Molecular Spectroscopy, Wroblewskiego 15, 93-590 Lodz, Poland.
Lodz University of Technology, Faculty of Chemistry, Institute of Applied Radiation Chemistry, Laboratory of Laser Molecular Spectroscopy, Wroblewskiego 15, 93-590 Lodz, Poland.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Dec 5;342:126465. doi: 10.1016/j.saa.2025.126465. Epub 2025 May 22.
Carotenoids represent a large group of chemical substances, and certain ones have demonstrated the ability to alter human metabolic pathways. Lycopene is an example of a carotenoid used for cancer prevention and therapy. Nonetheless, the precise impact of carotenoids on brain cancer cell metabolism and the functioning of individual organelles is still unclear. In this study, we suggested using Raman spectroscopy and imaging to analyse carotenoid metabolism at the single-cell level. This paper aimed to explore three representative carotenoid compounds to better understand their overall effects and their influence on brain cancer cells. Hence, utilizing Raman techniques, we analysed astrocytoma cells (CRL-1718 cell line) both without supplementation and supplemented with crocin, fucoxanthin, and lutein. Our Raman study suggests that the metabolism of cancer cells undergoes modifications upon carotenoid implementation. We showed that carotenoids affect lipids content (based on the intensity of the band at 1444 cm) and the redox state of cytochrome c inside a single cell (based on the intensity of the band at 1583 cm).
类胡萝卜素是一大类化学物质，其中某些类胡萝卜素已显示出改变人类代谢途径的能力。番茄红素就是一种用于癌症预防和治疗的类胡萝卜素。尽管如此，类胡萝卜素对脑癌细胞代谢和单个细胞器功能的确切影响仍不清楚。在本研究中，我们建议使用拉曼光谱和成像技术在单细胞水平分析类胡萝卜素代谢。本文旨在探索三种具有代表性的类胡萝卜素化合物，以更好地了解它们的总体作用及其对脑癌细胞的影响。因此，利用拉曼技术，我们分析了未添加以及添加了藏红花素、岩藻黄质和叶黄素的星形细胞瘤细胞（CRL-1718细胞系）。我们的拉曼研究表明，在实施类胡萝卜素后癌细胞的代谢会发生改变。我们发现类胡萝卜素会影响脂质含量（基于1444 cm处谱带的强度）和单个细胞内细胞色素c的氧化还原状态（基于1583 cm处谱带的强度）。
