Horie Sho, Okuda Chiaki, Yamashita Takatoshi, Watanabe Kenichi, Kuramochi Kouji, Hosokawa Masashi, Takeuchi Toshifumi, Kakuda Makiko, Miyashita Kazuo, Sugawara Fumio, Yoshida Hiromi, Mizushina Yoshiyuki
Laboratory of Food and Nutritional Sciences, Department of Nutritional Science, Kobe-Gakuin University, Nishi-ku, Kobe, Hyogo, 651-2180, Japan.
Lipids. 2010 Aug;45(8):713-21. doi: 10.1007/s11745-010-3449-5. Epub 2010 Jul 29.
In the screening of DNA polymerase (pol) inhibitor, we isolated lutein, a carotenoid, from the crude (unrefined) pressed oil of canola (low erucic acid rapeseed, Brassica napus L.). Commercially prepared carotenoids such as lutein (1), zeaxanthin (2), beta-cryptoxanthin (3), astaxanthin (4), canthaxanthin (5), beta-carotene (6), lycopene (7), capsanthin (8), fucoxanthin (9) and fucoxanthinol (10), were investigated for the inhibitory activities of pols. Compounds 1, 2 and 8 exhibited strong inhibition of the activities of mammalian pols beta and lambda, which are DNA repair- and/or recombination-related pols. On the other hand, all carotenoids tested had no influence on the activity of a mammalian pol alpha, which is a DNA replicative pol. Lutein (1) was the strongest pol inhibitor of mammalian pols beta and lambda in the prepared ten carotenoids tested, but did not influence of the activities of mammalian pols alpha, gamma, delta and epsilon. The tendency for pols beta and lambda inhibition by these carotenoids showed a positive correlation with the suppression of TPA (12-O-tetradecanoylphorbol-13-acetate)-induced inflammation. These results suggest that cold pressed unrefined canola/rapeseed oil, or other oils with high levels of lutein and other carotenoids, may be useful for their anti-inflammatory properties.
在筛选DNA聚合酶（pol）抑制剂的过程中，我们从油菜籽（低芥酸菜籽，甘蓝型油菜L.）的粗制（未精炼）压榨油中分离出了类胡萝卜素叶黄素。对市售的类胡萝卜素，如叶黄素（1）、玉米黄质（2）、β-隐黄质（3）、虾青素（4）、角黄素（5）、β-胡萝卜素（6）、番茄红素（7）、辣椒红素（8）、岩藻黄质（9）和岩藻黄醇（10），进行了pol抑制活性的研究。化合物1、2和8对哺乳动物的β和λ聚合酶活性表现出强烈抑制作用，这两种聚合酶与DNA修复和/或重组相关。另一方面，所有测试的类胡萝卜素对哺乳动物的α聚合酶活性均无影响，α聚合酶是一种DNA复制聚合酶。在所测试的十种类胡萝卜素中，叶黄素（1）是对哺乳动物β和λ聚合酶最强的抑制剂，但对哺乳动物α、γ、δ和ε聚合酶的活性没有影响。这些类胡萝卜素对β和λ聚合酶的抑制趋势与对佛波酯（12-O-十四酰佛波醇-13-乙酸酯）诱导的炎症的抑制呈正相关。这些结果表明，冷榨未精炼的油菜籽/菜籽油或其他富含叶黄素和其他类胡萝卜素的油，可能因其抗炎特性而具有实用价值。
