El Ghzaoui Chaimae, Neal Craig J, Dhungana Bijay, Nusrat Shanza, Fu Yifei, Kolanthai Elayaraja, Seal Sudipta
Advanced Materials Processing and Analysis Center (AMPAC), Department of Materials Science & Engineering, University of Central Florida, Orlando, FL, 32816, USA.
Nanoscience Technology Center, Biionix Cluster, College of Medicine, University of Central Florida, Orlando, FL, 32816, USA.
Anal Bioanal Chem. 2025 May 30. doi: 10.1007/s00216-025-05926-9.
Diabetes mellitus is a debilitating disease that can result in the formation of foot ulcers. Healing of these ulcers is additionally impeded, as a consequence of the condition, leading to the persistence of the wounds which can lead to additional ulceration and infection. miRNA203 expression has been correlated with the severity of diabetic wounds and can therefore function as a biomarker for wound health. In the presented study, an electrochemical platform sensor device was produced which allowed the detection of the miRNA203 analyte at concentrations from 1 μM down to 10 fM. The sensor was built using an electrode-bound single-strand DNA species, designed to promote selective hybridization to miRNA203, which was chemically functionalized to a redox-active copper-doped cerium oxide (CuCNP) particle formulation, mediating charge transfer to the electrode substrate. CuCNP synthesis was performed at varying copper contents (0, 5, 8, and 12 mol%) and each formulation was tested to identify an optimal composition for charge transfer in the sensor architecture.
糖尿病是一种使人衰弱的疾病，可导致足部溃疡的形成。由于这种病症，这些溃疡的愈合会受到进一步阻碍，导致伤口持续存在，进而可能引发更多溃疡和感染。miRNA203的表达与糖尿病伤口的严重程度相关，因此可作为伤口健康的生物标志物。在本研究中，制备了一种电化学平台传感器装置，该装置能够检测浓度从1 μM到10 fM的miRNA203分析物。该传感器使用与电极结合的单链DNA构建，设计用于促进与miRNA203的选择性杂交，其被化学功能化为氧化还原活性铜掺杂氧化铈（CuCNP）颗粒制剂，介导电荷转移到电极基板。在不同的铜含量（0、5、8和12摩尔％）下进行CuCNP合成，并对每种制剂进行测试，以确定传感器结构中电荷转移的最佳组成。
