Proportion of Immunoregulatory Cells in Inflammatory Infiltrate in Rheumatoid Arthritis Model and Its Correction by Antisense Oligonucleotides.

Autoimmune inflammation in rheumatoid arthritis is caused by various immunocompetent cells secreting a wide range of cytokines. Macrophages, CD4, and CD8 T cells are the most active in this process. We propose using antisense oligonucleotides (ASO) that block the synthesis of TNFα and IL-6. In the adjuvant-induced arthritis model, changes in the number of CD4, CD8, and CD68 cells in the inflammatory infiltrate after correction with ASO were assessed. The number of immunopositive inflammatory infiltrate cells in the area of bone damage was counted. CD8 cells turned out to be the most numerous. Blockade of IL-6 led to a statistically significant decrease in the number of CD4 cells. The use of ASO reduced the number of macrophages. The combination of ASO against IL-6 and TNFα administered by electrophoresis most effective.