Makalish T P, Pilyavskiy N N, Golovkin I O, Maslova G V
V. I. Vernadsky Crimean Federal University, Simferopol, Russia.
Bull Exp Biol Med. 2025 Apr;178(6):733-738. doi: 10.1007/s10517-025-06407-w. Epub 2025 May 30.
Autoimmune inflammation in rheumatoid arthritis is caused by various immunocompetent cells secreting a wide range of cytokines. Macrophages, CD4, and CD8 T cells are the most active in this process. We propose using antisense oligonucleotides (ASO) that block the synthesis of TNFα and IL-6. In the adjuvant-induced arthritis model, changes in the number of CD4, CD8, and CD68 cells in the inflammatory infiltrate after correction with ASO were assessed. The number of immunopositive inflammatory infiltrate cells in the area of bone damage was counted. CD8 cells turned out to be the most numerous. Blockade of IL-6 led to a statistically significant decrease in the number of CD4 cells. The use of ASO reduced the number of macrophages. The combination of ASO against IL-6 and TNFα administered by electrophoresis most effective.
类风湿关节炎中的自身免疫性炎症是由多种免疫活性细胞分泌多种细胞因子引起的。巨噬细胞、CD4和CD8 T细胞在这一过程中最为活跃。我们建议使用可阻断TNFα和IL-6合成的反义寡核苷酸（ASO）。在佐剂诱导的关节炎模型中，评估了用ASO校正后炎症浸润中CD4、CD8和CD68细胞数量的变化。对骨损伤区域免疫阳性炎症浸润细胞的数量进行了计数。结果发现CD8细胞数量最多。IL-6的阻断导致CD4细胞数量在统计学上显著减少。ASO的使用减少了巨噬细胞的数量。通过电泳给予抗IL-6和TNFα的ASO组合最为有效。
