Piaditis G P, Hodgkinson S C, McLean C, Lowry P J
J Immunoassay. 1985;6(3):299-319. doi: 10.1080/01971528508063035.
A liquid phase "two-site" immunoradiometric assay (IRMA) specific for human thyroid stimulating hormone (hTSH) is described. The assay is based on the simultaneous addition of affinity purified sheep anti hTSH IgG-I 125 and rabbit anti hTSH antiserum to standards and unknowns followed by 4h incubation at room temperature. The separation of free labelled sheep IgG-I125 from that bound to hTSH is achieved by the addition of sheep anti-rabbit IgG Fc fragment antiserum. The radiolabelled sheep anti-hTSH IgG-I 125 was pretreated with solid phase urinary postmenopausal gonadotropins to remove cross reaction with FSH and LH. The assay is specific for hTSH and no cross reaction with the other anterior pituitary glycoproteins or protein hormones has been found. In addition it is characterized by a wide operating range, rapid equilibration of reactants and high sensitivity (0.02 microU/ml). The precision of dose estimates was less than 10% between 0.25-2.5 microU/ml and less than 2.5% over the range 2.5-60 microU/ml.
本文描述了一种针对人促甲状腺激素（hTSH）的液相“双位点”免疫放射分析（IRMA）。该分析基于同时向标准品和未知样品中加入亲和纯化的羊抗hTSH IgG-I 125和兔抗hTSH抗血清，然后在室温下孵育4小时。通过加入羊抗兔IgG Fc片段抗血清，实现游离标记的羊IgG-I125与结合到hTSH上的部分的分离。放射性标记的羊抗hTSH IgG-I 125用固相绝经后尿促性腺激素进行预处理，以消除与FSH和LH的交叉反应。该分析对hTSH具有特异性，未发现与其他垂体前叶糖蛋白或蛋白质激素有交叉反应。此外，它具有较宽的工作范围、反应物快速平衡和高灵敏度（0.02微国际单位/毫升）的特点。在0.25 - 2.5微国际单位/毫升之间，剂量估计的精密度小于10%，在2.5 - 60微国际单位/毫升范围内小于2.5%。
