Sharma Neha, Simic Marija K, Davies Bethan K, Olesen Jacob Bastholm, Søe Kent, McDonald Michelle M
Clinical Cell Biology, Pathology Research Unit, University of Southern Denmark, Odense, Denmark.
Department of Molecular Medicine, University of Southern Denmark, Odense, Denmark.
Methods Mol Biol. 2025;2885:23-49. doi: 10.1007/978-1-0716-4306-8_2.
This chapter describes the isolation, expansion, staining, and imaging of osteoclasts from murine (MKS, BKD, and MMM) and human (NS, JBO, and KS) sources. We cover in detail both traditional and more modern methods of assessing osteoclast formation and function in vitro including advances in image acquisition and automated analyses. Importantly, we provide in-depth methods for human osteoclast culture systems, methods to assess human osteoclast function, and highlight potential methodological pitfalls and ways to overcome them. This collection of protocols provides a valuable resource for labs either initiating in vitro osteoclast assays or aiming to expand on traditional methods.
本章介绍了从小鼠（MKS、BKD和MMM）和人类（NS、JBO和KS）来源分离、扩增、染色和成像破骨细胞的方法。我们详细介绍了评估体外破骨细胞形成和功能的传统方法和更现代的方法，包括图像采集和自动分析方面的进展。重要的是，我们提供了人类破骨细胞培养系统的深入方法、评估人类破骨细胞功能的方法，并突出了潜在的方法陷阱以及克服这些陷阱的方法。这套实验方案为刚开始进行体外破骨细胞检测或希望扩展传统方法的实验室提供了宝贵的资源。
