啮齿动物破骨细胞培养物。

Rodent osteoclast cultures.

作者信息

Orriss Isabel R, Arnett Timothy R

机构信息

Department of Cell and Developmental Biology, University College London, London, UK.

出版信息

Methods Mol Biol. 2012;816:103-17. doi: 10.1007/978-1-61779-415-5_8.

DOI:10.1007/978-1-61779-415-5_8

PMID:22130925

Abstract

This chapter describes quantitative methods for isolating and culturing rodent osteoclasts on dentine, a bone-like, resorbable substrate. These techniques generate relatively large numbers of osteoclasts and allow the key processes of osteoclast formation and activation to be studied independently. A special focus will be on the role of extracellular pH, a critical factor in the control of osteoclast function.

摘要

本章介绍了在牙本质（一种类似骨且可吸收的基质）上分离和培养啮齿动物破骨细胞的定量方法。这些技术可产生相对大量的破骨细胞，并能独立研究破骨细胞形成和激活的关键过程。特别关注细胞外pH的作用，它是控制破骨细胞功能的关键因素。