Costa Samantha N, Chlebek Carolyn, Rosen Clifford J
Center for Molecular Medicine, MaineHealth Institute for Research, Scarborough, ME, USA.
Graduate School of Biomedical Science and Engineering, University of Maine, Orono, ME, USA.
Methods Mol Biol. 2025;2885:87-98. doi: 10.1007/978-1-0716-4306-8_5.
This chapter will present innovative methods for the isolation and characterization of mature, primary bone marrow adipocytes from rodents. The key advantage of this protocol is that it allows for ex vivo analyses of mature bone marrow adipocytes, which is a more direct approach than traditional in vitro studies. While the difficulty in isolating mature adipocytes from the heterogenous bone marrow population has precluded their characterization in many preclinical studies, we have optimized our methods to maximize the collection of alive, mature bone marrow adipocytes and minimize contamination of other bone marrow and immune cells.
本章将介绍从啮齿动物中分离和鉴定成熟原代骨髓脂肪细胞的创新方法。该方案的关键优势在于它允许对成熟骨髓脂肪细胞进行体外分析，这是一种比传统体外研究更直接的方法。虽然从异质性骨髓群体中分离成熟脂肪细胞的困难阻碍了它们在许多临床前研究中的鉴定，但我们已经优化了我们的方法，以最大限度地收集存活的成熟骨髓脂肪细胞，并尽量减少其他骨髓和免疫细胞的污染。
