骨细胞中转录调控的分析
Analysis of Transcriptional Regulation in Bone Cells.
作者信息
Jin Huilin, Sophocleous Antonia, Azfer Asim, Ralston Stuart H
机构信息
Synnovis, Molecular Genetics, 7th Floor, Borough Wing, Guys Hospital, UK, London, SE1 9RT.
School of Sciences, Department of Life Sciences, European University Cyprus, Nicosia, Cyprus.
出版信息
Methods Mol Biol. 2025;2885:247-269. doi: 10.1007/978-1-0716-4306-8_12.
Transcription is a process by which the rate of RNA synthesis is regulated. Here we describe the techniques for carrying out promoter-reporter assays: electrophoretic mobility shift assays, chromosome conformation capture (3C) assays, chromatin immunoprecipitation assays, and CRISPR-Cas9 assay-five commonly used methods for studying and altering gene transcription.
转录是一个调节RNA合成速率的过程。在此，我们描述了进行启动子-报告基因分析的技术：电泳迁移率变动分析、染色体构象捕获（3C）分析、染色质免疫沉淀分析以及CRISPR-Cas9分析——这是研究和改变基因转录的五种常用方法。
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