Jin Huilin, Sophocleous Antonia, Azfer Asim, Ralston Stuart H
Synnovis, Molecular Genetics, 7th Floor, Borough Wing, Guys Hospital, UK, London, SE1 9RT.
School of Sciences, Department of Life Sciences, European University Cyprus, Nicosia, Cyprus.
Methods Mol Biol. 2025;2885:247-269. doi: 10.1007/978-1-0716-4306-8_12.
Transcription is a process by which the rate of RNA synthesis is regulated. Here we describe the techniques for carrying out promoter-reporter assays: electrophoretic mobility shift assays, chromosome conformation capture (3C) assays, chromatin immunoprecipitation assays, and CRISPR-Cas9 assay-five commonly used methods for studying and altering gene transcription.
转录是一个调节RNA合成速率的过程。在此，我们描述了进行启动子-报告基因分析的技术：电泳迁移率变动分析、染色体构象捕获（3C）分析、染色质免疫沉淀分析以及CRISPR-Cas9分析——这是研究和改变基因转录的五种常用方法。
