Nguyen John V L, Malic Lidija, Nassif Christina, Da Fonte Dillon, Tabrizian Maryam
Department of Biomedical Engineering, McGill University, 3775 University Street, Montreal, QC H3A 2B4, Canada.
Department of Biomedical Engineering, McGill University, 3775 University Street, Montreal, QC H3A 2B4, Canada; Medical Devices, Life Sciences Division, National Research Council of Canada, 75 de Mortagne Boulevard, Boucherville, QC J4B 6Y4, Canada.
STAR Protoc. 2025 Jun 20;6(2):103859. doi: 10.1016/j.xpro.2025.103859. Epub 2025 May 30.
The generation of combinatorial oligonucleotide libraries is desirable for applications such as DNA aptamers, data storage, DNA origami, or synthetic genomes, but conventional libraries present challenges in detection and analysis. Synthesis of unique oligonucleotide sequences on magnetic nanoparticles would enhance the ability to manipulate, recover, and detect them. Here, we present a protocol for generating a scalable combinatorial oligonucleotide library on magnetic nanoparticles using split-and-pool synthesis. We then describe the process for preparing the library for conventional and next-generation sequencing (NGS) DNA sequencing. For complete details on the use and execution of this protocol, please refer to Nguyen et al..
组合寡核苷酸文库的生成对于DNA适配体、数据存储、DNA折纸或合成基因组等应用来说是很有必要的，但传统文库在检测和分析方面存在挑战。在磁性纳米颗粒上合成独特的寡核苷酸序列将增强对其进行操作、回收和检测的能力。在此，我们展示了一种使用拆分合并合成法在磁性纳米颗粒上生成可扩展组合寡核苷酸文库的方案。然后，我们描述了为传统测序和新一代测序（NGS）DNA测序准备文库的过程。有关此方案的使用和执行的完整详细信息，请参考阮等人的研究。
