Lai Yiling, Xu Jun, Wang Suqin, Li Hongbo, Yu Ruqin
Key Laboratory of Green Catalysis of Jiangxi Education Institutes, College of Chemistry and Materials, Jiangxi Normal University, Nanchang, 330022, China.
Key Laboratory of Green Catalysis of Jiangxi Education Institutes, College of Chemistry and Materials, Jiangxi Normal University, Nanchang, 330022, China.
Talanta. 2026 Jan 1;296:128383. doi: 10.1016/j.talanta.2025.128383. Epub 2025 May 25.
MicroRNA (miRNA) plays a crucial role in a great number of human cancers so that the detection of miRNA has been widely acknowledged as an effective strategy for the early discovery and diagnosis of cancer. Building upon conventional strand displacement amplification, the study was introduced a dual strand displacement process to enhance primer amplification efficiency. Additionally, we designed a lateral displacement assembly-based DNA nanostructure, expanding on traditional hairpin structures, and constructed a side-shifted hybrid chain reaction (SHCR) strategy for dual amplification of primer sequences. This approach yielded a three-way junction (3WJ) DNA nanostructure, providing optimal molecular recognition sites. The SHCR facilitated the assembly of fluorescent probes through a straightforward hybridization chain reaction. The target-triggered double strand displacement amplification (DSDA) exhibited high specificity for miRNA-let-7a with the rang from 50 fM to 50 nM, and the biosensor's limit of detection is 22 fM. Moreover, the sensor system performed exceptionally well in detecting real samples, such as cell lysates. Therefore, this work offers a novel strategy for early cancer screening.
微小RNA（miRNA）在大量人类癌症中起着关键作用，因此miRNA的检测已被广泛认为是癌症早期发现和诊断的有效策略。在传统链置换扩增的基础上，该研究引入了双链置换过程以提高引物扩增效率。此外，我们设计了一种基于侧向位移组装的DNA纳米结构，在传统发夹结构的基础上进行拓展，并构建了一种用于引物序列双重扩增的侧向位移杂交链反应（SHCR）策略。这种方法产生了一个三向连接（3WJ）DNA纳米结构，提供了最佳的分子识别位点。SHCR通过直接的杂交链反应促进了荧光探针的组装。目标触发的双链置换扩增（DSDA）对50 fM至50 nM范围内的miRNA-let-7a表现出高特异性，并且生物传感器的检测限为22 fM。此外，该传感器系统在检测实际样品（如细胞裂解物）方面表现出色。因此，这项工作为早期癌症筛查提供了一种新策略。
