Marks J L, Ax R L
J Dairy Sci. 1985 Aug;68(8):2078-82. doi: 10.3168/jds.S0022-0302(85)81070-5.
The binding of the glycosaminoglycan [3H] heparin to bull spermatozoa was compared with nonreturn rates of dairy bulls. Semen samples from five bulls above and five below an average 71% nonreturn rate were used. Samples consisted of first and second ejaculates on a single day collected 1 d/wk for up to 5 consecutive wk. Saturation binding assays using [3H] heparin were performed to quantitate the binding characteristics of each sample. Scatchard plot analyses indicated a significant difference in the binding affinity for [3H] heparin between bulls of high and low fertility. Dissociation constants were 69.0 and 119.3 pmol for bulls of high and low fertility, respectively. In contrast, the number of binding sites for [3H] heparin did not differ significantly among bulls. Differences in binding affinity of [3H] heparin to bull sperm might be used to predict relative fertility of dairy bulls.
将糖胺聚糖[3H]肝素与公牛精子的结合情况与奶牛的返情率进行了比较。使用了高于平均返情率71%的五头公牛和低于平均返情率71%的五头公牛的精液样本。样本包括在连续5周内每周1天采集的同一天的首次和第二次射精。使用[3H]肝素进行饱和结合试验，以定量每个样本的结合特性。Scatchard图分析表明，高生育力和低生育力公牛对[3H]肝素的结合亲和力存在显著差异。高生育力和低生育力公牛的解离常数分别为69.0和119.3 pmol。相比之下，[3H]肝素的结合位点数量在公牛之间没有显著差异。[3H]肝素与公牛精子结合亲和力的差异可能用于预测奶牛的相对生育力。
