Karatutlu Cumali, Kursunlu Ahmed Nuri
Department of Private Security and Protection, Suluova Vocational School, Amasya University, Amasya, Turkey; Department of Chemical Biological Radiology and Nuclear Defense, Health Sciences Institute University of Selcuk, Konya, Turkey.
Department of Chemistry, Faculty of Science, University of Selcuk, Konya, Turkey.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Dec 15;343:126506. doi: 10.1016/j.saa.2025.126506. Epub 2025 May 30.
The CBRN (chemical, biological, radiological and nuclear) materials caused to serious health risks that the effective detection of these agents is very important. Macrocyclic fluorescent sensors based on Pillar[5]arene bearing multi Bodipy units have been designed for toxic agents in the CBRN area. The prepared non-symmetric Pillar[5]arene derivative (Pillar[5]-Bodipys) was characterized by NMR, elemental analysis, FT-IR and melting point methods. The macrocyclic sensor exhibits a strong emission around 510 nm, which is used to monitor the interaction of toxic anions. Ion-binding experiments of the chemosensor have been examined in half-aqueous medium with a wide range of anions and the detection process is monitored by UV-vis and fluorescence spectroscopies. The results demonstrated that the chemosensor, Pillar[5]-Bodipys, has a sensitivity and selectivity toward toxic F ion. The fluorescence intensity of Pillar[5]-Bodipys quenched significantly in the presence of F anion and LOD was determined as 2.1x10 M. In the detection of F ion, Pillar[5]-Bodipys has also an ideal response time and good stability in a range of pH's. The biological imaging was examined for the detection of F ion by Pillar[5]-Bodipys, that the fluorescent macrocyclic sensor has a low toxicity. It can be successfully used in the recognition of F ions on living cells.
化学、生物、放射和核（CBRN）物质会引发严重的健康风险，因此有效检测这些物质非常重要。基于带有多个硼二吡咯单元的柱[5]芳烃设计了用于CBRN领域有毒物质的大环荧光传感器。通过核磁共振（NMR）、元素分析、傅里叶变换红外光谱（FT-IR）和熔点测定方法对制备的非对称柱[5]芳烃衍生物（柱[5]-硼二吡咯）进行了表征。该大环传感器在510nm左右表现出强烈的发射，用于监测有毒阴离子的相互作用。在半水介质中对该化学传感器与多种阴离子进行了离子结合实验，并通过紫外可见光谱和荧光光谱监测检测过程。结果表明，化学传感器柱[5]-硼二吡咯对有毒氟离子具有灵敏度和选择性。在氟阴离子存在下，柱[5]-硼二吡咯的荧光强度显著猝灭，检测限测定为2.1×10⁻⁶M。在检测氟离子时，柱[5]-硼二吡咯在一系列pH值范围内也具有理想的响应时间和良好的稳定性。对柱[5]-硼二吡咯检测氟离子的生物成像进行了研究，该荧光大环传感器具有低毒性。它可以成功用于活细胞上氟离子的识别。
