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蛋白质含量和pH值对由乳粉制成的微波膨化、可长期保存的奶酪泡芙零食特性的影响。

Impact of protein content and pH on the properties of microwaved, expanded, shelf-stable cheese puff snacks made from dairy powders.

作者信息

Pronschinske J M, Govindasamy-Lucey S, Jaeggi J J, Ibáñez R A, Johnson M E, Lucey J A

机构信息

Department of Food Science, University of Wisconsin-Madison, Madison, WI 53706.

Center for Dairy Research, University of Wisconsin-Madison, Madison, WI 53706.

出版信息

J Dairy Sci. 2025 Aug;108(8):8061-8075. doi: 10.3168/jds.2025-26654. Epub 2025 May 30.

DOI:10.3168/jds.2025-26654
PMID:40451576
Abstract

Certain cheeses (e.g., Mozzarella) can be stretched and molded when heated. If a cheese can stretch, perhaps its properties can also be modified to allow it to expand during microwave heating and drying to produce a crunchy, shelf-stable snack. To investigate this concept, we made a model system similar to pasteurized process cheese product (PPCP) that allowed us to vary 2 key parameters; protein (PN) content and the pH value, and to limit the meltability due to the high levels of intact casein. Cylinders of PPCP were microwaved to try to produce shelf-stable cheese puffs. Nine different PPCP treatments were made (n = 3) in a process cheese cooker using micellar casein powder, anhydrous milkfat, lactose, maltodextrin (MD), NaCl, trisodium citrate (TSC), citric acid (CA), and water. The MD addition was used to vary the PN content of PPCP from 30% (0% MD), 24% (∼8.3% MD), and 20% (∼16.8% MD). The use of MD allowed us to keep the moisture content constant in all the treatments. The pH values were varied from 5.7, 5.4, and 5.0, and the experimental design was a 3 × 3 factorial design of 3 PN levels and 3 pH levels. Ingredients were mixed in the process cheese cooker and then heated to 82°C for 2 min. All PPCP samples exhibited no melting (i.e., loss tangents <1) during heating when measured by small-strain oscillatory rheology. Buffering curves of the PPCP indicated that little of the indigenous colloidal calcium phosphate remained in the PPCP due to the addition of TSC and CA. After preliminary investigation, we found that expanded cheese could be formed from cylinders (9 mm in height × 13 mm in diameter) that were cut from PPCP and heated in a 900-W homestyle microwave oven for 45 to 70 s. These expanded puffs had a water activity of 0.24 ± 0.05. The puffs made from 30% PN samples expanded more (expansion of ∼440%) compared with the puffs made from 24% PN or 20% PN samples (expansions of ∼260% and ∼210%, respectively) when measured by glass bead displacement. In contrast, expansion was not significantly affected by the pH value of the PPCP base. After 1 wk of storage at room temperature, cheese puffs were evaluated by quantitative descriptive sensory analysis for textural attributes using trained panelists (n ≥8) based on a 15-point scale for texture. Crunchiness, tooth packing, and hardness attributes were lower for puffs made with 30% PN samples than the values for those made with 20% PN samples. Tooth cling, evaluated on a 6-point scale, was highest for puffs made with 20% PN samples (∼4.5). Puff texture was unaffected by the pH value. Scanning electron microscopy showed thicker cell walls for the puffs made from 24% and 20% PN samples when compared with 30% PN samples. Our model PPCP system could puff and expand, but the MD added to adjust (reduce) the PN content negatively affected expansion. The PN content of the PPCP was more important than the pH value for its expansion properties. A lack of melt during heating appeared to help facilitate expansion when PPCP was heated in a microwave.

摘要

某些奶酪(如马苏里拉奶酪)在加热时可以拉伸和塑形。如果一种奶酪能够拉伸,那么或许其特性也可以被改变,使其在微波加热和干燥过程中膨胀,从而制成一种酥脆、耐储存的零食。为了探究这一概念,我们制作了一个类似于巴氏杀菌加工干酪制品(PPCP)的模型系统,该系统使我们能够改变两个关键参数:蛋白质(PN)含量和pH值,并由于完整酪蛋白含量高而限制其可熔性。将PPCP圆柱体进行微波处理,试图制作出耐储存的奶酪泡芙。在一个加工干酪蒸煮器中,使用胶束酪蛋白粉、无水乳脂肪、乳糖、麦芽糊精(MD)、NaCl、柠檬酸三钠(TSC)、柠檬酸(CA)和水,制作了9种不同的PPCP处理(n = 3)。添加MD是为了使PPCP的PN含量从30%(0% MD)、24%(约8.3% MD)和20%(约16.8% MD)变化。使用MD使我们能够在所有处理中保持水分含量恒定。pH值分别为5.7、5.4和5.0,实验设计为3个PN水平和3个pH水平的3×3析因设计。将原料在加工干酪蒸煮器中混合,然后加热至82°C并保持2分钟。通过小应变振荡流变学测量发现,所有PPCP样品在加热过程中均未出现熔化(即损耗角正切<1)。PPCP的缓冲曲线表明,由于添加了TSC和CA,PPCP中几乎没有残留的原生胶体磷酸钙。经过初步研究,我们发现可以从PPCP切下的圆柱体(高9毫米×直径13毫米)制成膨胀奶酪,并在900瓦的家用微波炉中加热45至70秒。这些膨胀的泡芙水分活度为0.24±0.05。通过玻璃珠位移测量发现,与由24% PN或20% PN样品制成的泡芙(膨胀率分别约为260%和210%)相比,由30% PN样品制成的泡芙膨胀得更多(膨胀率约为440%)。相比之下,PPCP底料的pH值对膨胀没有显著影响。在室温下储存1周后,由经过培训的评判员(n≥8)基于15分制的质地标准,通过定量描述性感官分析对奶酪泡芙的质地属性进行评估。由30% PN样品制成的泡芙的酥脆度、牙齿填充度和硬度属性低于由20% PN样品制成的泡芙。以6分制评估的牙齿附着力方面,由20% PN样品制成的泡芙最高(约4.5)。泡芙质地不受pH值影响。扫描电子显微镜显示,与30% PN样品相比,由24%和20% PN样品制成的泡芙的细胞壁更厚。我们的模型PPCP系统能够膨胀,但为调节(降低)PN含量而添加的MD对膨胀有负面影响。PPCP的PN含量对其膨胀特性比pH值更重要。当PPCP在微波炉中加热时,加热过程中不熔化似乎有助于促进膨胀。

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