Wei Zhen, Li Jinjian, Wang Xue, Wang Xu, Zhao Dexi
Medical Intensive Care Unit, Hospital of Changchun University of Chinese Medicine, Changchun, Jilin, 130021, China.
Neurosurgery Department, Hospital of Changchun University of Chinese Medicine, Changchun, Jilin, 130021, China.
J Thromb Thrombolysis. 2025 Jun 1. doi: 10.1007/s11239-025-03120-3.
A growing body of evidence points to a strong link between ischemic stroke and the gut microbiome. Given the wide diversity present in gut microbiota, this research intends to employ advanced and thorough data to investigate the causative relationship between gut microbiota and ischemic stroke. We performed a two-sample study using Mendelian randomization to clarify the causal connection between gut microbiota and ischemic stroke. The GISCOME network encompassed 6,021 individuals with ischemic stroke, primarily of European descent. A total of 473 gut microbial taxa were extracted from the genome-wide association study catalog. The research involved a forward Mendelian randomization approach(gut microbiota as exposure, ischemic stroke as outcome). A variety of analytical techniques were applied, including inverse variance weighting, Weighted Median, MR-Egger, Weighted Mode, and Simple Mode. Following this, a sensitivity analysis was performed to confirm the reliability of our findings. Rats underwent treatment using a middle cerebral artery occlusion model, and after 7 days, stool samples were collected for 16s sequencing to assess changes in gut microbiota and to compare these with the Mendelian randomization results. Our analysis suggests a potential causal association between gut microbiota and ischemic stroke. Through forward causal analysis, relationships of causality between 20 different gut microbial taxa and ischemic stroke were unveiled. Findings from 16s sequencing indicated that there was an overlap of 6 gut microbial taxa with the results of Mendelian randomization. The results of our research indicate a direct link between gut microbiota and ischemic stroke, offering possible direction for upcoming clinical trials.
越来越多的证据表明缺血性中风与肠道微生物群之间存在紧密联系。鉴于肠道微生物群具有广泛的多样性，本研究旨在运用先进且全面的数据来探究肠道微生物群与缺血性中风之间的因果关系。我们采用孟德尔随机化方法进行了一项双样本研究，以阐明肠道微生物群与缺血性中风之间的因果联系。GISCOME网络涵盖了6021名缺血性中风患者，主要为欧洲血统。从全基因组关联研究目录中总共提取了473种肠道微生物分类群。该研究采用了正向孟德尔随机化方法（将肠道微生物群作为暴露因素，缺血性中风作为结果）。应用了多种分析技术，包括逆方差加权法、加权中位数法、MR-Egger法、加权众数法和简单众数法。随后，进行了敏感性分析以确认我们研究结果的可靠性。使用大脑中动脉闭塞模型对大鼠进行治疗，7天后收集粪便样本进行16S测序，以评估肠道微生物群的变化，并将其与孟德尔随机化结果进行比较。我们的分析表明肠道微生物群与缺血性中风之间可能存在因果关联。通过正向因果分析，揭示了20种不同肠道微生物分类群与缺血性中风之间的因果关系。16S测序结果表明，有6种肠道微生物分类群与孟德尔随机化结果存在重叠。我们的研究结果表明肠道微生物群与缺血性中风之间存在直接联系，为即将开展的临床试验提供了可能的方向。
