Mackenzie I, Howlett J, Ranasinghe A
J Invest Dermatol. 1985 Oct;85(4):314-8. doi: 10.1111/1523-1747.ep12276899.
The use of organ cultures of mouse ear skin for examining mechanisms of stratum corneum shedding has been investigated. The formation and loss of stratum corneum cells was assessed by electron microscopic autoradiography and from counts of stratum corneum cell layers taken from sections of plastic-embedded specimens. Using media supplemented with cortisone, there was a linear accumulation of stratum corneum cell layers and the rate of epidermal cell formation corresponded approximately to that in vivo. Cells that accumulated at the stratum corneum surface were loose and could consistently be removed by treatment with detergent and ultrasonication indicating the persistence of shedding mechanisms in vitro.
已对使用小鼠耳部皮肤器官培养物来研究角质层脱落机制进行了调查。通过电子显微镜放射自显影以及对取自塑料包埋标本切片的角质层细胞层数计数来评估角质层细胞的形成和流失。使用添加了可的松的培养基时，角质层细胞层呈线性积累，表皮细胞形成速率大致与体内情况相符。在角质层表面积累的细胞松散，通过用去污剂处理和超声处理能够持续将其去除，这表明体外存在脱落机制。
