Ranasinghe A W, Wertz P W, Downing D T, Mackenzie I C
J Invest Dermatol. 1986 Feb;86(2):187-90. doi: 10.1111/1523-1747.ep12284246.
An organ culture system has been used to examine differences in the lipid compositions of materials derived from cohesive and desquamated mouse ear stratum corneum. Within this culture system, skin explants display rates of cell replication and differentiation comparable to those observed in vivo for up to 2 weeks and, during this period, loosened or dishesive material accumulates at the surface. Lipid compositions were determined for both intact and loosened stratum corneum derived from cultured skin and also for freshly prepared stratum corneum. In all 3 cases, the profiles of the nonpolar lipids and the ceramides were essentially the same; some of the nonpolar lipids appeared to be of sebaceous origin. The only changes detected upon desquamation were reductions of cholesteryl sulfate and a second unidentified lipid of similar polarity. Cholesteryl sulfate constitutes 4-5% of the polar lipid in fresh stratum corneum or stratum corneum from organ culture. This is reduced to 0.4% in the desquamated material which accumulates in the culture system. The unidentified lipid decreases from 1-2% of the polar lipid in intact fresh or cultured stratum corneum to 0.1% in the desquamated material. The possible function of cholesteryl sulfate in corneocyte cohesion is discussed.
一种器官培养系统已被用于研究源自粘性和脱落的小鼠耳角质层的材料在脂质组成上的差异。在这个培养系统中，皮肤外植体在长达2周的时间内表现出与体内观察到的相似的细胞复制和分化速率，并且在此期间，松散或易分离的物质在表面积累。测定了来自培养皮肤的完整和松散角质层以及新鲜制备的角质层的脂质组成。在所有这三种情况下，非极性脂质和神经酰胺的谱图基本相同；一些非极性脂质似乎来源于皮脂腺。脱落后检测到的唯一变化是硫酸胆固醇酯和另一种极性相似的未鉴定脂质的减少。硫酸胆固醇酯在新鲜角质层或器官培养的角质层中的极性脂质中占4 - 5%。在培养系统中积累的脱落物质中，这一比例降至0.4%。未鉴定脂质在完整的新鲜或培养角质层的极性脂质中占1 - 2%，在脱落物质中降至0.1%。文中讨论了硫酸胆固醇酯在角质形成细胞粘附中的可能功能。
