Xu Feiyang, Cui Ting, Long Zhendong, Shen Haidong, Xie Hong, Liu Hongfu, Xie Jianfei, Yang Kai, Tao Yugui
School of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, People's Republic of China.
State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection & School for Radiological and Interdisciplinary Sciences (RADX), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, Jiangsu 215123, People's Republic of China.
ACS Infect Dis. 2025 Jun 13;11(6):1707-1718. doi: 10.1021/acsinfecdis.5c00249. Epub 2025 Jun 1.
The development of drug-resistant biofilms has resulted in treatment failures for multidrug resistant (MDR) bacteria, presenting a substantial challenge to global public health. Consequently, the development of antibacterial agents has become an urgent priority. To overcome the limitations of poor penetration and inadequate efficacy of single-modal treatments for MDR bacterial biofilm infections, this study developed a Ce6/SFP nanocomposite system utilizing silk fibroin and polydopamine. The nanoparticles, fabricated via a desolvation-in situ polymerization method, demonstrated pH-responsive drug release properties. When subjected to an 808 nm NIR laser, the material exhibited a photothermal conversion efficiency of 29.3%. Furthermore, when paired with singlet oxygen produced through 660 nm excitation, it was highly effective in eliminating MDR and MDR . The synergistic effect of photothermal therapy (PTT) and photodynamic therapy (PDT) disrupted the stability of the cell membrane and enhanced the antibacterial efficacy. Experimental results showed that the photothermal effect increased biofilm permeability, significantly improving Ce6 penetration depth. Moreover, in vivo treatments exhibited accelerated wound healing. Biocompatibility assessments revealed a hemolysis rate below 5% and no pathological damage to major organs. This study proposes a synergistic strategy to effectively address the challenges posed by biofilms and bacterial resistance.
耐药生物膜的形成导致多重耐药（MDR）细菌的治疗失败，给全球公共卫生带来了巨大挑战。因此，开发抗菌剂已成为当务之急。为了克服单模式治疗对MDR细菌生物膜感染穿透力差和疗效不足的局限性，本研究利用丝素蛋白和聚多巴胺开发了一种Ce6/SFP纳米复合系统。通过去溶剂化原位聚合法制备的纳米颗粒表现出pH响应性药物释放特性。当用808nm近红外激光照射时，该材料的光热转换效率为29.3%。此外，当与660nm激发产生的单线态氧结合时，它对消除MDR和MDR非常有效。光热疗法（PTT）和光动力疗法（PDT）的协同作用破坏了细胞膜的稳定性，提高了抗菌效果。实验结果表明，光热效应增加了生物膜的通透性，显著提高了Ce6的穿透深度。此外，体内治疗显示伤口愈合加快。生物相容性评估显示溶血率低于5%，主要器官无病理损伤。本研究提出了一种协同策略，以有效应对生物膜和细菌耐药性带来的挑战。
