Yang Yunxia, Zhang Hongmei, Zhang Xueqing, Shen Shuyan, Wu Baojuan, Peng Dexin, Yin Jie, Wang Yanqing
School of Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng 224007, China.
Jiangsu Province Engineering Research Center of Agricultural Breeding Pollution Control and Resource, Yancheng Teachers University, Yancheng 224007, China.
Gels. 2025 Aug 21;11(8):672. doi: 10.3390/gels11080672.
Bacterial infections cause serious problems associated with wound treatment and serious complications, leading to serious threats to the global public. Bacterial resistance was mainly attributed to the formation of biofilms and their protective properties. Hydrogels suitable for irregular surfaces with effective antibacterial activity have attracted extensive attention as potential materials. In this study, a succinoglycan-riclin-zinc-phthalocyanine-based composite (RL-Zc) hydrogel was synthesized through an amine reaction within an hour. The hydrogel was characterized via FT-IR, SEM, and rheology analysis, exhibiting an elastic solid gel state stably. The hydrogel showed large inhibition circles on as well as under near-infrared irradiation (NIR). RL-Zc hydrogel exhibited positively charged surfaces and possessed a superior penetrability toward bacterial biofilm. Furthermore, RL-Zc hydrogel generated abundant single oxygen and mild heat rapidly, resulting in disrupted bacterial biofilm as well as amplified antibacterial effectiveness. A metabolomics analysis confirmed that RL-Zc hydrogel induced a metabolic disorder in bacteria, which resulted from phospholipid metabolism and oxidative stress metabolism related to biofilm disruption. Hence, this study provided a potential phototherapy for biofilm-induced bacterial resistance.
细菌感染会引发与伤口治疗相关的严重问题及严重并发症，对全球公众构成严重威胁。细菌耐药性主要归因于生物膜的形成及其保护特性。具有有效抗菌活性、适用于不规则表面的水凝胶作为潜在材料受到了广泛关注。在本研究中，通过胺反应在一小时内合成了一种基于琥珀聚糖-蓖麻毒素-锌酞菁的复合（RL-Zc）水凝胶。通过傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）和流变学分析对该水凝胶进行了表征，其稳定呈现出弹性固体凝胶状态。该水凝胶在近红外辐射（NIR）下对[具体细菌1]和[具体细菌2]均显示出较大的抑菌圈。RL-Zc水凝胶表面带正电荷，对细菌生物膜具有优异的穿透性。此外，RL-Zc水凝胶能迅速产生大量单线态氧和温和热量，导致细菌生物膜被破坏，抗菌效果增强。代谢组学分析证实，RL-Zc水凝胶诱导细菌发生代谢紊乱，这是由与生物膜破坏相关的磷脂代谢和氧化应激代谢引起的。因此，本研究为生物膜诱导的细菌耐药性提供了一种潜在的光疗方法。
