体内脓肿定位与中性粒细胞体外迁移及黏附之间的关系。

Relation between in vivo abscess localization and in vitro migration and adherence of neutrophils.

作者信息

Bamberger D M, Gerding D N, Bettin K M, Elson M K, Forstrom L A

出版信息

J Infect Dis. 1985 Nov;152(5):903-12. doi: 10.1093/infdis/152.5.903.

DOI:10.1093/infdis/152.5.903

Abstract

Rabbit peripheral blood and glycogen-stimulated peritoneal neutrophils were labeled with [111In]indium oxine and transfused intravenously into recipient rabbits with experimental abdominal abscesses due to Staphylococcus aureus. Peritoneal neutrophils harvested 4 hr after glycogen infusion localized within the abscesses to a greater extent than did peripheral blood neutrophils (P less than .002). In an in vitro chemotaxis under-agarose assay, peripheral blood neutrophils had greater random migration (P less than .002) and directed migration (P less than .01) than did peritoneal cells. In an in vitro glass slide adherence assay, peritoneal neutrophils were more adherent than were blood neutrophils (P less than .05). The discrepancy between in vivo and in vitro findings may be due to the increased adherence of peritoneal neutrophils. Glycogen-stimulated peritoneal neutrophils have been exposed in vivo to C5a, which is known to decrease migration and increase adherence in vitro of polymorphonuclear neutrophils; consequently, in vivo exposure of neutrophils to C5a may mean in vitro migration data may be misleading in predicting results in vivo.

摘要

用[111铟]氧嗪标记兔外周血和糖原刺激的腹腔中性粒细胞，然后静脉输注到因金黄色葡萄球菌导致实验性腹部脓肿的受体兔体内。糖原输注4小时后收集的腹腔中性粒细胞在脓肿内的定位程度比外周血中性粒细胞更大（P<0.002）。在体外琼脂糖下趋化试验中，外周血中性粒细胞比腹腔细胞具有更大的随机迁移（P<0.002）和定向迁移（P<0.01）。在体外玻片黏附试验中，腹腔中性粒细胞比血液中性粒细胞更具黏附性（P<0.05）。体内和体外研究结果之间的差异可能是由于腹腔中性粒细胞黏附性增加所致。糖原刺激的腹腔中性粒细胞在体内已暴露于C5a，已知C5a可降低体外多形核中性粒细胞的迁移并增加其黏附性；因此，中性粒细胞在体内暴露于C5a可能意味着体外迁移数据在预测体内结果时可能会产生误导。