Li Churong, Zhao Biao, Xiong Jing, Li Linjie, Pang Dalong, Unger Keith, Jung Mira, Lyu Jiahua, Kuang Hao, Liang Long, Li Tao, Chen Long, Bai Hansong
Radiotherapy Center, Radiation Oncology Key Laboratory of Sichuan Province, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, University of Electronic Science and Technology of China, Chengdu, China.
Non-Coding RNA and Drug Discovery Key Laboratory of Sichuan Province, School of Basic Medical Sciences, Chengdu Medical College, Chengdu, China.
J Ginseng Res. 2025 May;49(3):260-270. doi: 10.1016/j.jgr.2025.01.004. Epub 2025 Jan 27.
Ginsenoside Rg5 possesses potent anti-oxidative, anti-inflammatory, and cytoprotective properties. This study explored the protective effects of ginsenoside Rg5 on radiation-induced pulmonary microvascular endothelial cells (PMECs) injury and the associated molecular mechanisms.
C57BL/6 mice were used for studies and primary human PMECs (PPMECs) were utilized as models. Mice with or without ginsenoside Rg5 pretreatment were irradiated by varying doses. Lung tissues were analyzed for histopathological changes and the expression of endothelial markers. , PPMECs were irradiated with or without ginsenoside Rg5 pretreatment and analyzed for apoptosis, oxidative stress, mitochondrial function, and endothelial barrier integrity.
Ginsenoside Rg5 pretreatment attenuated radiation-induced acute lung damage, preserved endothelial cell junction integrity, and maintained endothelial barrier function . , ginsenoside Rg5 significantly reduced IR-induced oxidative stress, apoptosis, and mitochondrial dysfunction in PPMECs. Ginsenoside Rg5 suppressed radiation-induced Mfn2 acetylation and proteasomal degradation via Sirt1-mediated deacetylation, thereby preserving mitochondrial dynamics and integrity. The protective effects of ginsenoside Rg5 on the integrity of mitochondrial and endothelial tight junction proteins and barrier function were also Sirt1-dependent.
Ginsenoside Rg5 exerts a protective effect against radiation-induced endothelial injury by modulating mitochondrial dynamics and function, as well as maintaining endothelial barrier integrity, in a Sirt1-dependent manner.
人参皂苷Rg5具有强大的抗氧化、抗炎和细胞保护特性。本研究探讨了人参皂苷Rg5对辐射诱导的肺微血管内皮细胞（PMECs）损伤的保护作用及其相关分子机制。
使用C57BL/6小鼠进行研究，并将原代人肺微血管内皮细胞（PPMECs）用作模型。对接受或未接受人参皂苷Rg5预处理的小鼠进行不同剂量的照射。分析肺组织的组织病理学变化和内皮标志物的表达。此外，对接受或未接受人参皂苷Rg5预处理的PPMECs进行照射，并分析其凋亡、氧化应激、线粒体功能和内皮屏障完整性。
人参皂苷Rg5预处理减轻了辐射诱导的急性肺损伤，保留了内皮细胞连接完整性，并维持了内皮屏障功能。此外，人参皂苷Rg5显著降低了辐射诱导的PPMECs中的氧化应激、凋亡和线粒体功能障碍。人参皂苷Rg5通过Sirt1介导的去乙酰化抑制辐射诱导的Mfn2乙酰化和蛋白酶体降解，从而维持线粒体动力学和完整性。人参皂苷Rg5对线粒体和内皮紧密连接蛋白完整性及屏障功能的保护作用也是Sirt1依赖性的。
人参皂苷Rg5通过以Sirt1依赖性方式调节线粒体动力学和功能以及维持内皮屏障完整性，对辐射诱导的内皮损伤发挥保护作用。
