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含MAM结构域蛋白2（MAMDC2）影响人胃癌的侵袭和转移。

MAM Domain Containing 2 (MAMDC2) Affects Invasion and Metastasis of Human Gastric Cancer.

作者信息

Shen Jiaofeng, Gao Guangyu, Liu Songtao

机构信息

Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, People's Republic of China.

Department of Ultrasound, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, People's Republic of China.

出版信息

Onco Targets Ther. 2025 May 26;18:679-693. doi: 10.2147/OTT.S516982. eCollection 2025.

DOI:10.2147/OTT.S516982
PMID:40453636
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12124315/
Abstract

BACKGROUND

Gastric cancer (GC) is the fifth most prevalent cancer worldwide and the fourth leading cause of cancer-related mortality. MAM Domain Containing 2 (MAMDC2) has been involved in many cancers. However, the impact of MAMDC2 on gastric cancer was unclear. This study aimed to investigate the role and mechanism of MAMDC2 in gastric cancer.

METHODS

Differential genes in gastric cancer are analyzed by GEO, TCGA, MSigDB database, R-packet limma, and Wilcoxon test. Survival analysis is performed through the R package survival. Construct a PPI network through the STRING database. Enrichment analysis is performed by Metascape. The infiltration level of gastric cancer immune cells is calculated by CIBERSORT. In addition, the expression of MAMDC2 was analyzed by immunohistochemistry and quantitative real-time polymerase chain reaction (RT-qPCR). siMAMDC2 was used to knock down the specific gene. Cell counting kit-8 (CCK-8) assay, colony formation assay, and cell migration were applied to evaluate the function of MAMDC2 in gastric cancer cells.

RESULTS

In the present study, we revealed a significant upregulation of MAMDC2 in gastric cancer tissues and cells. Knocking down MAMDC2 inhibited the proliferation and migration of gastric cancer cells, while overexpression of MAMDC2 produced the opposite results. Furthermore, MAMDC2 may be an independent factor in poor prognosis in gastric cancer patients.

CONCLUSION

These results illustrated that MAMDC2 promoted the proliferation and migration of gastric cancer cells. The newly identified MAMDC2 provides novel insight into the pathogenesis of gastric cancer.

摘要

背景

胃癌（GC）是全球第五大常见癌症，也是癌症相关死亡的第四大主要原因。含MAM结构域蛋白2（MAMDC2）已涉及多种癌症。然而，MAMDC2对胃癌的影响尚不清楚。本研究旨在探讨MAMDC2在胃癌中的作用及机制。

方法

通过GEO、TCGA、MSigDB数据库、R包limma和Wilcoxon检验分析胃癌中的差异基因。通过R包survival进行生存分析。通过STRING数据库构建蛋白质-蛋白质相互作用（PPI）网络。由Metascape进行富集分析。通过CIBERSORT计算胃癌免疫细胞的浸润水平。此外，通过免疫组织化学和定量实时聚合酶链反应（RT-qPCR）分析MAMDC2的表达。使用siMAMDC2敲低特定基因。应用细胞计数试剂盒-8（CCK-8）测定、集落形成测定和细胞迁移来评估MAMDC2在胃癌细胞中的功能。

结果

在本研究中，我们发现MAMDC2在胃癌组织和细胞中显著上调。敲低MAMDC2可抑制胃癌细胞的增殖和迁移，而MAMDC2的过表达则产生相反的结果。此外，MAMDC2可能是胃癌患者预后不良的独立因素。

结论

这些结果表明，MAMDC2促进了胃癌细胞的增殖和迁移。新鉴定的MAMDC2为胃癌的发病机制提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a077/12124315/6b4c4e86fdff/OTT-18-679-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a077/12124315/c0973ff0747c/OTT-18-679-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a077/12124315/77666cf9588b/OTT-18-679-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a077/12124315/847e3f6014be/OTT-18-679-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a077/12124315/d7fc1ee196b8/OTT-18-679-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a077/12124315/be3a38f5e871/OTT-18-679-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a077/12124315/0351c05ae8bc/OTT-18-679-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a077/12124315/6b4c4e86fdff/OTT-18-679-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a077/12124315/c0973ff0747c/OTT-18-679-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a077/12124315/77666cf9588b/OTT-18-679-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a077/12124315/847e3f6014be/OTT-18-679-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a077/12124315/d7fc1ee196b8/OTT-18-679-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a077/12124315/be3a38f5e871/OTT-18-679-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a077/12124315/0351c05ae8bc/OTT-18-679-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a077/12124315/6b4c4e86fdff/OTT-18-679-g0007.jpg

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