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丛枝菌根真菌的分子系统发育、形态学、菌根共生及推测分布()

Molecular phylogeny, morphology, mycorrhizal symbiosis, and putative distribution of the arbuscular mycorrhizal fungus ().

作者信息

Yamato Masahide, Ohmae Muneyuki, Orihara Takamichi, Kusakabe Ryota, Goto Bruno Tomio, Błaszkowski Janusz

机构信息

a Faculty of Education, Chiba University.

b Hokken Co. Ltd.

出版信息

Mycoscience. 2024 Oct 9;65(6):270-277. doi: 10.47371/mycosci.2024.08.002. eCollection 2025.

Abstract

We collected in Japan five sporocarpic specimens morphologically identical to those of , a recently described species. Although 18S-ITS-28S nuc rDNA sequences obtained from these sporocarps showed high sequence variability, phylogenetic analyses based on 18S-ITS-28S, the largest subunit of the RNA polymerase II () gene, and concatenated sequences of the two loci convincingly demonstrated the identity of these sporocarps to . Importantly, the 18S-ITS-28S+ analyses highlighted the key role of sequences in reconstructing the phylogenies of taxa with strongly divergent rDNA sequences. Upon inoculation with sporocarpic spores, formed mycorrhiza with arbuscules and vesicles, which was not confirmed in the original description of the species. Comparisons of 18S sequences with DNA sequences available in a public database indicated that is a cosmopolitan species and is mainly associated with plants in natural habitats such as grasslands, shrublands, and forests. Phylogenetic analyses also confirmed the autonomy of , another known species of the genus whose sporocarps were originally found in Brazil.

摘要

我们在日本采集了5个孢子果标本,其形态与最近描述的一种物种的标本相同。尽管从这些孢子果获得的18S - ITS - 28S核rDNA序列显示出高度的序列变异性,但基于18S - ITS - 28S、RNA聚合酶II()基因的最大亚基以及这两个位点的串联序列进行的系统发育分析令人信服地证明了这些孢子果与该物种相同。重要的是,18S - ITS - 28S +分析突出了序列在重建rDNA序列差异很大的类群系统发育中的关键作用。用孢子果孢子接种后,形成了具有丛枝和泡囊的菌根,这在该物种的原始描述中未得到证实。将18S序列与公共数据库中可用的DNA序列进行比较表明,是一种世界性物种,主要与草原、灌木丛和森林等自然栖息地中的植物相关。系统发育分析还证实了的自主性,是该属另一个已知物种,其孢子果最初在巴西被发现。

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