Ramadan Rana, El-Sherbini El-Said, Elsayed Gehad, El-Adl Mohamed
Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Department of Basic Veterinary Science, Faculty of Veterinary Medicine, Delta University for Science and Technology, Mansoura, Egypt.
Open Vet J. 2025 Apr;15(4):1823-1835. doi: 10.5455/OVJ.2025.v15.i4.35. Epub 2025 Apr 30.
Topiramate (TPM) is commonly used for migraine prophylaxis; nonetheless, it is linked to nephrotoxicity, which mostly results from oxidative stress and inflammatory mechanisms.
This study aimed to assess the preventive effects of gallic acid (GA) and furosemide (Lasix) against renal damage caused by TPM in rats.
Sixty male albino rats were categorized into six groups: control, TPM-only, TPM + Lasix, Lasix-only, TPM + GA, and GA-only. The investigation lasted 60 days, evaluating renal function, oxidative stress indicators, and histological and immunohistochemical alterations.
The TPM-treated group exhibited considerable renal impairment, as indicated by increased levels of creatinine, urea, blood urea nitrogen, and interleukin (IL)-6, as well as reduced activity of antioxidant enzymes [catalase (CAT), superoxide dismutase]. Histopathological examination revealed tubular necrosis and inflammation, while immunohistochemistry results showed elevated expression of caspase3 and IL-6. The co-administration of GA or Lasix mitigated these effects. The TPM + GA group exhibited improved kidney function, less oxidative stress, and enhanced histological structure, underscoring GA's powerful antioxidant and anti-inflammatory characteristics. Likewise, Lasix exerted protective effects by alleviating TPM-induced kidney injury.
These data highlight the therapeutic efficacy of GA and Lasix against TPM-induced nephrotoxicity, indicating their clinical relevance in addressing drug-induced kidney damage.
托吡酯（TPM）常用于偏头痛预防；然而，它与肾毒性有关，这主要是由氧化应激和炎症机制引起的。
本研究旨在评估没食子酸（GA）和呋塞米（速尿）对TPM诱导的大鼠肾损伤的预防作用。
将60只雄性白化大鼠分为六组：对照组、仅TPM组、TPM + 速尿组、仅速尿组、TPM + GA组和仅GA组。研究持续60天，评估肾功能、氧化应激指标以及组织学和免疫组化改变。
TPM治疗组表现出明显的肾损伤，表现为肌酐、尿素、血尿素氮和白细胞介素（IL）-6水平升高，以及抗氧化酶[过氧化氢酶（CAT）、超氧化物歧化酶]活性降低。组织病理学检查显示肾小管坏死和炎症，而免疫组化结果显示caspase3和IL-6表达升高。GA或速尿的联合使用减轻了这些影响。TPM + GA组肾功能改善，氧化应激减轻，组织结构增强，突出了GA强大的抗氧化和抗炎特性。同样，速尿通过减轻TPM诱导的肾损伤发挥保护作用。
这些数据突出了GA和速尿对TPM诱导的肾毒性的治疗效果，表明它们在解决药物性肾损伤方面的临床相关性。
