Chen Kuan-Hsun, Wang Wei-Yu, Hu Cho-Chun, Chiu Tai-Chia
Department of Applied Science, National Taitung University, 369, Section 2, University Road, Taitung 950309, Taiwan.
ACS Omega. 2025 May 13;10(20):20435-20443. doi: 10.1021/acsomega.5c00575. eCollection 2025 May 27.
A facile sensing assay was demonstrated for the selective and sensitive detection of thiram, using malic acid-functionalized silver nanoparticles (MA-AgNPs) as colorimetric probes. The factors affecting the synthesis of the MA-AgNPs were optimized and characterized by ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy, transmission electron microscopy, and X-ray photoelectron spectroscopy. The MA-AgNPs were spherical, with an average diameter of 7.8 nm and a maximum absorbance at 390 nm. Further, the stability of the storage time, buffer pH, and salinity of the MA-AgNPs was investigated. The MA-AgNPs were employed to detect thiram in environmental samples. Good linearity of the calibration curve was achieved (0.1-1.0 ppm), with of 0.998 and a limit of detection (LOD) of 0.009 ppm. Additionally, the assay displayed good anti-interference toward thiram over other coexisting pesticides. More importantly, a smartphone-assisted colorimetric assay integrated with test paper was established for thiram detection, with an LOD of 0.71 ppm. This work shows the potential feasibility of the rational design for thiram detection in actual samples.
本文展示了一种简便的传感分析方法，用于选择性和灵敏地检测福美双，该方法使用苹果酸功能化的银纳米颗粒（MA-AgNPs）作为比色探针。通过紫外可见光谱、傅里叶变换红外光谱、透射电子显微镜和X射线光电子能谱对影响MA-AgNPs合成的因素进行了优化和表征。MA-AgNPs呈球形，平均直径为7.8 nm，在390 nm处有最大吸收峰。此外，还研究了MA-AgNPs的储存时间、缓冲液pH值和盐度的稳定性。MA-AgNPs用于检测环境样品中的福美双。校准曲线具有良好的线性（0.1-1.0 ppm），相关系数为0.998，检测限（LOD）为0.009 ppm。此外，该分析方法对福美双显示出良好的抗干扰能力，优于其他共存农药。更重要的是，建立了一种与试纸相结合且由智能手机辅助的比色分析方法用于福美双检测，检测限为0.71 ppm。这项工作表明了在实际样品中合理设计福美双检测方法的潜在可行性。
