Sun Zhiyu, Ollier Claire, Rancz Adrienn, Thienpont Batiste, Grohe Kristof, Becker Lukas, Purea Armin, Engelke Frank, Wegner Sebastian, Sturgis James, Polenova Tatyana, Le Marchand Tanguy, Pintacuda Guido
Centre de RMN à Très Hauts Champs de Lyon (UMR 5082), CNRS, Ecole Normale Supérieure de Lyon and Université Clade Bernard Lyon 1, Villeurbanne 69100, France.
LISM UMR 7255, CNRS and Aix-Marseille University, Marseille 13402, France.
J Am Chem Soc. 2025 Jun 11;147(23):19433-19437. doi: 10.1021/jacs.5c02466. Epub 2025 Jun 2.
We present ultrafast magic-angle spinning (MAS) at 160 kHz in solid-state nuclear magnetic resonance (NMR), demonstrating unprecedented spectral quality and coherence lifetimes in proton-detected experiments on biomolecular systems. By optimizing experimental and sample conditions and utilizing advanced filling and handling tools, we achieve superior resolution on both microcrystalline and membrane-reconstituted proteins, paving the way for new applications in structural biology.
我们展示了在固态核磁共振（NMR）中以160 kHz进行的超快魔角旋转（MAS），在生物分子系统的质子检测实验中展现出了前所未有的光谱质量和相干寿命。通过优化实验和样品条件，并利用先进的填充和处理工具，我们在微晶和膜重构蛋白上均实现了卓越的分辨率，为结构生物学的新应用铺平了道路。
