Jie Yin, Feng Shengjie, Zheng Dianfeng, Feng Naijie, Zhou Hang, Huang Xixin, Huang Anqi, Zhang Rongjun, Meng Fengyan
College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, Guangdong, China.
South China Center of National Saline-Tolerant Rice Technology Innovation Center, Zhanjiang, Guangdong, China.
PLoS One. 2025 Jun 2;20(6):e0284734. doi: 10.1371/journal.pone.0284734. eCollection 2025.
Rice (Oryza sativa L.) is a glycophyte that can be easily poisoned by salt toxicity. 1',4'-trans-diol-ABA is a crucial precursor in the biosynthesis of abscisic acid (ABA) in fungi that may alleviate salt stress in plants; however, the role of this compound on salt tolerance in rice has not yet been reported. In the present study, 1',4'-trans-diol-ABA was applied to rice seedling varieties "Huanghuazhan" (HHZ) and "Xiangliangyou 900" (XLY900) to investigate the resulting tolerance against saline stress. Results demonstrated that salinity stress reduced the growth parameters of seedlings of two rice cultivars, made lower photosynthesis and fluorescence parameters. The salt treatment also increased the concentration and Na + content of malondialdehyde (MDA) of two rice cultivars, and decreased the catalase (CAT) activity, ascorbyl peroxidase (APX) and peroxidase (POD) activity, ascorbic acid (AsA) and glutathione (GSH) of rice seedlings of two varieties. The results showed that the exogenous addition of 1',4'-trans-diol-ABA decreased salt-induced reactive oxygen species (ROS) accumulation and oxidative stress and improved the morphological characteristics of both rice cultivars, compared to cultivars without the addition of 1',4'-trans-diol-ABA. Furthermore, changes in different phytohormones in rice seedlings were triggered by 1',4'-trans-diol-ABA treatment. Simultaneously, the Na + /K + ratios in the shoots and roots of treated plants were lower than in the non-treated plants, which may be due to the higher K + concentration observed in treated plants under saline water irrigation conditions. In conclusion, this study revealed that 1',4'-trans-diol-ABA may perform a significant function in increasing crop salt-stress tolerance.
水稻（Oryza sativa L.）是一种糖生植物，容易受到盐毒害。1',4'-反式二醇脱落酸是真菌中脱落酸（ABA）生物合成的关键前体，可能减轻植物的盐胁迫；然而，该化合物对水稻耐盐性的作用尚未见报道。在本研究中，将1',4'-反式二醇脱落酸应用于水稻幼苗品种“黄华占”（HHZ）和“湘两优900”（XLY900），以研究其对盐胁迫的耐受性。结果表明，盐胁迫降低了两个水稻品种幼苗的生长参数，降低了光合作用和荧光参数。盐处理还增加了两个水稻品种丙二醛（MDA）的浓度和Na +含量，降低了过氧化氢酶（CAT）活性、抗坏血酸过氧化物酶（APX）和过氧化物酶（POD）活性，以及两个品种水稻幼苗的抗坏血酸（AsA）和谷胱甘肽（GSH）。结果表明，与未添加1',4'-反式二醇脱落酸的品种相比，外源添加1',4'-反式二醇脱落酸减少了盐诱导的活性氧（ROS）积累和氧化胁迫，改善了两个水稻品种的形态特征。此外，1',4'-反式二醇脱落酸处理引发了水稻幼苗中不同植物激素的变化。同时，处理植株地上部和根部的Na + /K + 比值低于未处理植株，这可能是由于在盐水灌溉条件下处理植株中观察到较高的K + 浓度。总之，本研究表明1',4'-反式二醇脱落酸在提高作物耐盐胁迫方面可能发挥重要作用。
