Van Thao Nguyen, McAllister Jaime, Alfaro Andrea C, Ngo Nha T, Mundy Craig
NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, Viet Nam; Aquaculture Biotechnology Research Group, School of Science, Auckland University of Technology, Auckland, New Zealand.
IMAS Fisheries and Aquaculture Centre, College of Science and Engineering, University of Tasmania, Taroona, Tasmania, Australia.
Comp Biochem Physiol A Mol Integr Physiol. 2025 Sep;307:111883. doi: 10.1016/j.cbpa.2025.111883. Epub 2025 May 31.
Transport stress significantly influences the well-being and survival of abalone, yet the underlying metabolic changes and physiological responses associated with this stress remain poorly understood. To gain comprehensive insights into the metabolic changes and physiological responses of abalone (Haliotis rubra) under transport stress, we conducted a targeted liquid chromatography-mass spectrometry (LC-MS) metabolomics investigation on haemolymph and gill samples collected at different time points, including post-harvesting (pre-transport), post-transport and post-immersion (water holding period) and post-live exporting transport. The results revealed 143 and 141 metabolites that were significantly different among sampling times in haemolymph and gill, respectively. Notably, most of the metabolite differences occurred between the post-transport and post-immersion times, which indicate the strong impacts of transport stress on abalone metabolism. Interestingly, certain metabolites, such as lactic acid, succinic acid, L-hydroxyglutaric acid, uric acid, and myo-inositol, showed time-dependent increases during transport, suggesting their potential as stress biomarkers in abalone. Moreover, abalone that were acclimatized in holding tanks exhibited lesser metabolic changes compared to non-acclimatized, despite both groups being transported with oxygen supply. This highlights the significance of acclimatization and oxygen supply in reducing stress for abalone during transport. Enrichment analysis on abalone samples at 24 h post-transport compared to 96 h post-immersion identified 12 significantly impacted pathways in haemolymph and 34 pathways in gill tissues, indicating a range of metabolic disturbances in transported abalone, such as energy-related pathways, amino acid metabolisms, carbohydrate metabolisms, vitamin metabolisms, oxidative stress, and others. These results offer valuable insights into the physiological responses of abalone to transport stress, guiding improved transport practices to ensure good quality of products arriving at their market destinations.
运输应激对鲍鱼的健康和存活有显著影响，然而与这种应激相关的潜在代谢变化和生理反应仍知之甚少。为了全面了解运输应激下鲍鱼（红鲍）的代谢变化和生理反应，我们对在不同时间点采集的血淋巴和鳃样本进行了靶向液相色谱 - 质谱（LC - MS）代谢组学研究，这些时间点包括收获后（运输前）、运输后、浸泡后（暂养期）和活体出口运输后。结果显示，血淋巴和鳃中分别有143种和141种代谢物在采样时间之间存在显著差异。值得注意的是，大多数代谢物差异发生在运输后和浸泡后之间，这表明运输应激对鲍鱼代谢有强烈影响。有趣的是，某些代谢物，如乳酸、琥珀酸、L - 羟基戊二酸、尿酸和肌醇，在运输过程中呈现出随时间增加的趋势，表明它们有可能作为鲍鱼应激生物标志物。此外，尽管两组都在有氧气供应的情况下运输，但在暂养池中适应过的鲍鱼与未适应的相比，代谢变化较小。这突出了适应和氧气供应在减少鲍鱼运输应激方面的重要性。对运输后24小时与浸泡后96小时的鲍鱼样本进行的富集分析表明，血淋巴中有12条显著受影响的途径，鳃组织中有34条途径，这表明运输后的鲍鱼存在一系列代谢紊乱，如能量相关途径、氨基酸代谢、碳水化合物代谢、维生素代谢、氧化应激等。这些结果为鲍鱼对运输应激的生理反应提供了有价值的见解，有助于指导改进运输方法，以确保到达市场目的地的产品质量良好。
