Cutaia Alessandra, Prosperi Sofia, Zanoni Camilla, Alberti Giancarla
Department of Chemistry, University of Pavia, via Taramelli, Pavia, 12-27100, Italy.
Sci Rep. 2025 Jun 2;15(1):19293. doi: 10.1038/s41598-025-04802-x.
The increasing need for rapid and cost-effective methods to quantify furan compounds in food has spurred the development of in-field analysis techniques. Addressing this demand, we present a novel, low-cost disposable sensor for the direct voltammetric detection of key furanic aldehydes - 2-furaldehyde (2-FAL), 5-hydroxymethyl-2-furaldehyde (5-HMF), and 5-methyl-2-furaldehyde (5-MFAL) - in Italian honey samples. Our approach utilizes a screen-printed voltammetric cell with a carbon-ink working electrode modified by an electropolymerized molecularly imprinted polypyrrole film (e-MIP), with 2-FAL as the template. The electropolymerization conditions for the e-MIP film were optimized using a Face-centered Central Composite Design. The sensor's analytical performance was evaluated using square wave voltammetry (SWV) in 2-FAL standard aqueous solutions (0.1 M phosphate buffer at pH 8), and its applicability was demonstrated by quantifying furaldehydes in real honey samples via the standard additions method. Interference and recovery tests confirmed the sensor's good performance, suggesting its potential for routine furaldehyde analysis in this food matrix.
对快速且经济高效的食品中呋喃化合物定量方法的需求不断增加，这推动了现场分析技术的发展。为满足这一需求，我们展示了一种新型低成本一次性传感器，用于直接伏安法检测意大利蜂蜜样品中的关键呋喃醛——2-呋喃甲醛（2-FAL）、5-羟甲基-2-呋喃甲醛（5-HMF）和5-甲基-2-呋喃甲醛（5-MFAL）。我们的方法采用了一种丝网印刷伏安电池，其碳墨工作电极由以2-FAL为模板的电聚合分子印迹聚吡咯膜（e-MIP）修饰。使用面心中央复合设计对e-MIP膜的电聚合条件进行了优化。在2-FAL标准水溶液（pH 8的0.1 M磷酸盐缓冲液）中使用方波伏安法（SWV）评估了传感器的分析性能，并通过标准加入法对实际蜂蜜样品中的呋喃醛进行定量，证明了其适用性。干扰和回收率测试证实了该传感器的良好性能，表明其在这种食品基质中进行常规呋喃醛分析的潜力。
