Hsiao Ming-Yen, Lin Yu-Ling, Lin Meng-Ting, Liao Wei-Hao, Chen Wen-Shiang, Wu Chueh-Hung
Department of Physical Medicine and Rehabilitation, College of Medicine, National Taiwan University, Taipei, Taiwan.
Department of Physical Medicine and Rehabilitation, National Taiwan University Hospital, Taipei, Taiwan.
CNS Neurosci Ther. 2025 Jun;31(6):e70451. doi: 10.1111/cns.70451.
This study aimed to investigate the regional modulation effect of low-intensity focused ultrasound (LIFU) on cerebrospinal fluid (CSF) influx dynamics, focusing on the differential changes between hemispheres in a mouse model of unilateral ischemic stroke.
LIFU was administered with a commercial transducer at the unilateral M1 cortex of mice using specific parameters (0.5 MHz center frequency, 250 kHz pulse repetition frequency, 300 ms burst duration, 2 ms pulse length, 50% intraburst duty factor, 2 s interstimulation interval, spatial peak temporal average intensities of 0.5 W/cm, and a total sonication duration of 10 min). The regional CSF influx was observed through in vivo macroscopy and brain section imaging in a photothrombotic stroke model, both with and without LIFU.
Localized LIFU stimulation at the M1 region triggered a rapid CSF influx, peaking 20-30 min poststimulation. Following photothrombotic stroke, a marked reduction in CSF influx was observed in both ipsilesional and contralesional MCA regions, most notably on Days 1 and 3, with a return to baseline by Day 7. However, daily contralesional LIFU stimulation for 7 days effectively restored CSF influx on both sides.
Daily contralesional LIFU stimulation restores impaired CSF influx bilaterally after stroke, suggesting potential therapeutic implications for stroke-related pathophysiology.
本研究旨在探讨低强度聚焦超声（LIFU）对脑脊液（CSF）流入动力学的区域调节作用，重点关注单侧缺血性中风小鼠模型中半球间的差异变化。
使用商用换能器，以特定参数（中心频率0.5 MHz、脉冲重复频率250 kHz、脉冲串持续时间300 ms、脉冲长度2 ms、脉冲串内占空比50%、刺激间隔2 s、空间峰值时间平均强度0.5 W/cm以及总超声处理持续时间10 min）对小鼠单侧M1皮质进行LIFU治疗。在光血栓性中风模型中，通过体内宏观成像和脑切片成像观察有无LIFU时的区域脑脊液流入情况。
M1区域的局部LIFU刺激引发脑脊液快速流入，在刺激后20 - 30分钟达到峰值。光血栓性中风后，同侧和对侧大脑中动脉区域的脑脊液流入均显著减少，最明显的是在第1天和第3天，到第7天恢复至基线水平。然而，连续7天对侧LIFU刺激可有效恢复双侧脑脊液流入。
中风后连续7天对侧LIFU刺激可双侧恢复受损的脑脊液流入，提示其对中风相关病理生理学具有潜在治疗意义。
