Kshirsagar Shirin, Deshmukh Indraja S, Samel Deepali, Talathi Ved M, Meshram Rishikesh, Razdan Priyanka, Gupta Seema
Department of Conservative Dentistry and Endodontics, Yogita Dental College and Hospital, Khed, IND.
Department of Conservative Dentistry and Endodontics, Swargiya Dadasaheb Kalmegh Smruti Dental College and Hospital, Nagpur, IND.
Cureus. 2025 May 3;17(5):e83404. doi: 10.7759/cureus.83404. eCollection 2025 May.
Reducing microleakage is crucial for maintaining the long-term efficacy of composite restorations. The selection of the cavity liner may affect both the marginal seal and the structural integrity of the restoration. This study aimed to assess and compare microleakage in class II composite restorations using two distinct light-cured pulp capping agents as cavity liners, analyzed under a stereomicroscope.
Class II cavities with standardized dimensions were prepared for 60 noncarious human mandibular premolars. All the specimens were randomly allocated to three distinct groups, each containing 20 samples. In group 1 (n = 20), a self-etch bonding agent (Scotchbond Universal, 3M, Minnesota, USA) was uniformly applied to the entire cavity using an applicator tip and subsequently light-cured for 20 seconds. The cavities were then restored using composite resin (Filtek Z350 XT, 3M, Minnesota, USA) and light-cured for 30 seconds. In group 2 (n = 20), an approximately 0.8-mm-thick layer of TheraCal LC (Bisco Inc., Illinois, USA) was applied to the axial wall as a cavity liner. Group 3 (n = 20) involved the application of an approximately 0.8-mm-thick layer of Bio-LCB (Essential Dental Systems, New Jersey, USA) on the axial wall as a cavity liner. All tooth surfaces, except the 2-mm-wide area surrounding the edges of each restoration, were sealed with two nail polish coats. This was followed by a 48-hour immersion in 2% methylene blue dye. The teeth were then rinsed and sectioned longitudinally in the mesiodistal direction, coincident with the center of the restoration, using a slow-speed water-cooled diamond disc. To assess microleakage, the two tooth parts exhibiting dye penetration were examined under a stereomicroscope at 20x magnification (RSMr-6 series; Radical Scientific Equipment Pvt. Ltd., Haryana, India). Intergroup comparisons were performed using one-way analysis of variance (ANOVA), followed by post-hoc analysis using Tukey's test.
Statistically significant differences were observed between the groups (p < 0.05). Group 1 exhibited the highest mean microleakage values (3.35 ± 0.59), group 2 showed a moderate reduction in microleakage with a mean value of 2.30 ± 1.17, and group 3 had the lowest mean microleakage of 1.15 ± 0.93. Post-hoc analysis revealed significant differences between the groups. Groups 2 and 3 showed better results than group 1 (p < 0.05).
It can be concluded that Bio-LCB, as a light-cure pulp capping agent, showed minimal microleakage when used as a cavity liner in class II composite restorations.
减少微渗漏对于维持复合树脂修复体的长期疗效至关重要。窝洞衬层的选择可能会影响修复体的边缘封闭性和结构完整性。本研究旨在评估和比较使用两种不同的光固化护髓剂作为窝洞衬层的Ⅱ类复合树脂修复体的微渗漏情况，并在体视显微镜下进行分析。
为60颗无龋的人类下颌前磨牙制备具有标准化尺寸的Ⅱ类窝洞。所有标本随机分为三组，每组20个样本。在第1组（n = 20）中，使用涂抹头将自酸蚀粘结剂（Scotchbond Universal，3M，美国明尼苏达州）均匀涂抹于整个窝洞，随后光固化20秒。然后使用复合树脂（Filtek Z350 XT，3M，美国明尼苏达州）修复窝洞并光固化30秒。在第2组（n = 20）中，在轴壁上涂抹一层约0.8毫米厚的TheraCal LC（Bisco公司，美国伊利诺伊州）作为窝洞衬层。第3组（n = 20）是在轴壁上涂抹一层约0.8毫米厚的Bio-LCB（Essential Dental Systems，美国新泽西州）作为窝洞衬层。除每个修复体边缘周围2毫米宽的区域外，所有牙齿表面均用两层指甲油密封。随后将其在2%亚甲蓝染料中浸泡48小时。然后冲洗牙齿，并使用低速水冷金刚石切割片沿近远中方向在修复体中心处纵向切割。为了评估微渗漏情况，在体视显微镜下以20倍放大倍数（RSMr - 6系列；Radical Scientific Equipment Pvt. Ltd.，印度哈里亚纳邦）检查显示染料渗透的两个牙齿部分。组间比较采用单因素方差分析（ANOVA），随后使用Tukey检验进行事后分析。
各组之间观察到统计学上的显著差异（p < 0.05）。第1组的平均微渗漏值最高（3.35 ± 0.59），第2组微渗漏有适度降低，平均值为2.30 ± 1.17，第3组的平均微渗漏最低，为1.15 ± 0.93。事后分析显示各组之间存在显著差异。第2组和第3组的结果优于第1组（p < 0.05）。
可以得出结论，Bio-LCB作为光固化护髓剂，在Ⅱ类复合树脂修复体中用作窝洞衬层时显示出最小的微渗漏。
