Division of Cariology, Operative Dentistry and Endodontics, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
Yamamoto Dental Clinic, Takarazuka, Japan.
Int Endod J. 2017 Dec;50 Suppl 2:e73-e82. doi: 10.1111/iej.12737. Epub 2017 Jan 16.
To evaluate the Ca -releasing, alkalizing and apatite-like surface precipitate-forming abilities of a prototype tricalcium silicate cement, which was mainly composed of synthetically prepared tricalcium silicate and zirconium oxide radiopacifier.
The prototype tricalcium silicate cement, white ProRoot MTA (WMTA) and TheraCal LC (a light-cured resin-modified calcium silicate-filled material) were examined. The chemical compositions were analysed with a wavelength-dispersive X-ray spectroscopy electron probe microanalyser with an image observation function (SEM-EPMA). The pH and Ca concentrations of water in which the set materials had been immersed were measured, and the latter was assessed with the EDTA titration method. The surface precipitates formed on the materials immersed in phosphate-buffered saline (PBS) were analysed with SEM-EPMA and X-ray diffraction (XRD). Kruskal-Wallis tests followed by Mann-Whitney U-test with Bonferroni correction were used for statistical analysis (α = 0.05).
The prototype cement contained Ca, Si and Zr as major elemental constituents, whereas it did not contain some metal elements that were detected in the other materials. The Ca concentrations and pH of the immersion water samples exhibited the following order: WMTA = prototype cement > TheraCal LC (P < 0.05). All three materials produced Ca- and P-containing surface precipitates after PBS immersion, and the precipitates produced by TheraCal LC displayed lower Ca/P ratios than those formed by the other materials. XRD peaks corresponding to hydroxyapatite were detected in the precipitates produced by the prototype cement and WMTA.
The prototype tricalcium silicate cement exhibited similar Ca -releasing, alkalizing and apatite-like precipitate-forming abilities to WMTA. The Ca -releasing, alkalizing and apatite-like precipitate-forming abilities of TheraCal LC were lower than those of the other materials.
评估主要由合成制备的硅酸三钙和氧化锆放射阻射剂组成的原型硅酸三钙水泥的释钙、碱化和类磷灰石表面沉淀形成能力。
对原型硅酸三钙水泥、白色 ProRoot MTA（WMTA）和 TheraCal LC（一种光固化树脂改性硅酸钙填充材料）进行了研究。采用具有图像观察功能的波长相散 X 射线光谱电子探针微分析仪（SEM-EPMA）分析化学成分。测量浸泡在设定材料中的水的 pH 值和 Ca 浓度，并采用 EDTA 滴定法评估后者。用 SEM-EPMA 和 X 射线衍射（XRD）分析浸泡在磷酸盐缓冲液（PBS）中的材料形成的表面沉淀物。采用 Kruskal-Wallis 检验，然后采用 Mann-Whitney U 检验，并用 Bonferroni 校正进行统计分析（α=0.05）。
原型水泥含有 Ca、Si 和 Zr 等主要元素成分，而其他材料中则未检测到一些金属元素。浸出水中 Ca 浓度和 pH 值的顺序为：WMTA=原型水泥>TheraCal LC（P<0.05）。所有三种材料在 PBS 浸泡后都产生了含有 Ca 和 P 的表面沉淀物，TheraCal LC 产生的沉淀物的 Ca/P 比值低于其他材料形成的沉淀物。在原型水泥和 WMTA 产生的沉淀中检测到对应于羟基磷灰石的 XRD 峰。
原型硅酸三钙水泥表现出与 WMTA 相似的释钙、碱化和类磷灰石沉淀形成能力。TheraCal LC 的释钙、碱化和类磷灰石沉淀形成能力低于其他材料。
