Powell R J, Leyland A M, Pound J D, Bossingham D H
J Rheumatol. 1985 Jun;12(3):427-31.
A simple radioimmunoassay suitable for the routine estimation of IgG rheumatoid factors (IgG RF) has been developed. This involves determination of the amount of IgG RF binding to highly purified human IgG Fc on microtiter plates using a radiolabelled F(ab')2 preparation of an antihuman IgG Fd antiserum. A correction for nonspecific binding is made by subtracting sample reactivity with bovine serum albumin. Significantly elevated IgG RF levels were found only in patients with symmetrical peripheral erosive polyarthritis and in some patients with "mixed connective tissue disease."
已开发出一种适用于常规检测IgG类风湿因子（IgG RF）的简单放射免疫测定法。该方法包括使用抗人IgG Fd抗血清的放射性标记F(ab')2制剂，在微量滴定板上测定与高度纯化的人IgG Fc结合的IgG RF量。通过减去样品与牛血清白蛋白的反应性来校正非特异性结合。仅在患有对称性外周侵蚀性多关节炎的患者和一些患有“混合性结缔组织病”的患者中发现IgG RF水平显著升高。
