Wang Shurong, Sun Qing, Zhao Yang, Huo Bo
Biomechanics Lab, Department of Mechanics, School of Aerospace Engineering, Beijing Institute of Technology, Beijing 100081, PR China.
Institute of Artificial Intelligence in Sports, Capital University of Physical Education and Sports, Beijing 100191, PR China.
Mechanobiol Med. 2023 Aug 9;2(1):100012. doi: 10.1016/j.mbm.2023.100012. eCollection 2024 Mar.
Cell migration is an important biological process regulated by mechanical stimulation, which leads to intracellular calcium response. Cell migration are dependent on the distribution and dynamic changes of intracellular calcium concentration. However, the temporal relation among mechanical stimulation, cell migration, and intracellular calcium distribution remains unclear. In this study, unidirectional flow and oscillatory flow were applied on osteoclast precursor RAW264.7 cells. The parameters of cell migration under fluid flow and intracellular calcium distribution along the migration or flow direction were calculated. Experimental results suggest the cells to adjust the [Ca] distribution in the migration direction is independent of flow application or the reverse of flow direction, but the [Ca] distribution in the flow direction is determined by the [Ca] distribution-adjusting ability of cells and flow stimulation. Blocking calcium signaling pathways, namely, mechanosensitive cation-selective channels, phospholipase C, and endoplasmic reticulum, and removing extracellular calcium inhibited cell migration along the flow direction and the gradient distribution of intracellular calcium. This study provided insights into the mechanism of flow-induced cell migration and quantitative data for the recruitment of osteoclast precursors targeting the location of bone resorption.
细胞迁移是一个受机械刺激调节的重要生物学过程，机械刺激会引发细胞内钙反应。细胞迁移依赖于细胞内钙浓度的分布和动态变化。然而，机械刺激、细胞迁移和细胞内钙分布之间的时间关系仍不清楚。在本研究中，对破骨细胞前体RAW264.7细胞施加单向流和振荡流。计算了流体流动下细胞迁移的参数以及沿迁移或流动方向的细胞内钙分布。实验结果表明，细胞在迁移方向上调节[Ca]分布与施加流动或流动方向的反转无关，但流动方向上的[Ca]分布由细胞的[Ca]分布调节能力和流动刺激决定。阻断钙信号通路，即机械敏感阳离子选择性通道、磷脂酶C和内质网，并去除细胞外钙，会抑制细胞沿流动方向的迁移以及细胞内钙的梯度分布。本研究为流动诱导细胞迁移的机制提供了见解，并为破骨细胞前体向骨吸收部位募集提供了定量数据。
