Husák Tomáš, Čadková Zuzana, Máca Ondřej, Kouba Marek, Klimková Zdeňka, Sehnal Richard, Nápravníková Jana, Hrabětová Václava, Jankovská Ivana, Vadlejch Jaroslav, Langrová Iva
Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czechia.
Department of Pathology and Parasitology, State Veterinary Institute Prague, Prague, Czechia.
Front Vet Sci. 2025 May 19;12:1571082. doi: 10.3389/fvets.2025.1571082. eCollection 2025.
Larval stages of carnivore cestodes in rodents have been extensively studied for decades, primarily based on morphological indicators. Numerous datasets, particularly from Central Europe, exist on this topic. Traditionally, the shape, number, and size of hooks have been key distinguishing features. However, recent findings suggest that morphology alone may not provide accurate identification. In this study, rodent livers from various habitats across five regions of the Czech Republic were examined for the presence of taeniid larval cestodes.
A total of 2,934 rodent specimens were collected using snap traps between 2014 and 2022. Taeniid metacestodes detected in these intermediate hosts were subsequently subjected to species determination through molecular (targeting the cytochrome oxidase subunit I gene) and morphological examinations.
The following cestodes were identified using molecular methods: (found in , , , and ), (in , , and ), and (in and . ). Sequencing of 55 PCR-positive isolates revealed 13 haplotypes of . , one of . and four of . . Further, we also identified co-infection by more metacestode species or haplotypes. No rodent liver samples tested were positive for or sensu stricto, either through morphological or molecular identification methods. Furthermore, no other cryptic species were detected during this study.
This study presents the first comprehensive molecular data on the aforementioned zoonotic cestode species in the Czech Republic. The findings demonstrate the importance of using molecular techniques for Taeniidae species determination, as morphological methods may lead to erroneous classifications. Furthermore, this research highlights the importance of accurate diagnostic techniques, enabling the development of effective prevention and control strategies by verifying host-parasite relationships.
几十年来，主要基于形态学指标对啮齿动物体内食肉动物绦虫的幼虫阶段进行了广泛研究。关于这个主题存在大量数据集，特别是来自中欧的数据集。传统上，钩子的形状、数量和大小一直是关键的鉴别特征。然而，最近的研究结果表明，仅靠形态学可能无法提供准确的鉴定。在本研究中，对捷克共和国五个地区不同栖息地的啮齿动物肝脏进行了检查，以确定是否存在带科幼虫绦虫。
2014年至2022年期间，使用 snap 陷阱共收集了2934只啮齿动物标本。随后，对这些中间宿主体内检测到的带科中绦期幼虫进行分子（靶向细胞色素氧化酶亚基I基因）和形态学检查，以确定其种类。
使用分子方法鉴定出以下绦虫：（在、、、和中发现），（在、和中），以及（在和中）。对55个PCR阳性分离株进行测序，发现了13种的单倍型。，的一种单倍型和的四种单倍型。此外，我们还鉴定出了多种中绦期幼虫种类或单倍型的共感染情况。通过形态学或分子鉴定方法，未检测到任何啮齿动物肝脏样本对或严格意义上的呈阳性。此外，在本研究中未检测到其他隐性物种。
本研究提供了捷克共和国上述人畜共患绦虫物种的首个全面分子数据。研究结果表明，使用分子技术进行带科物种鉴定非常重要，因为形态学方法可能导致错误分类。此外，本研究强调了准确诊断技术的重要性，通过验证宿主 - 寄生虫关系，有助于制定有效的预防和控制策略。
